Jennifer Lake's Blog

May 22, 2020

Counterrevolution: Evolving

*                                                                       

The best antidote to fascist Techno-feudalism is living naturally with rational technology!

*

Here’s real biology from medical doctor experts who care about health:

RNA viruses

Dr. Nancy Banks, author of AIDS, Opium, Diamonds and Empire, from her 2015 interview with Sofia Smallstorm:

When a cell does not have enough electrons to function it begins to lose its ability to communicate with other parts of the cell –and this happens over time [not suddenly]…and means there is some toxicity or some deficiency that [isn’t] recognized… Cancer cells [for example] no longer receive the correct signals…  AIDS is defined as 29 different diseases –but AID [Acquired Immune Deficiency] is a malfunction of a certain kind of white cell… Even in virology they know that RNA viruses are not pathogenic… The theory of HIV is that the virus is in the CD4 [T-cells]…killing them… but the virus has not been found in the decreasing [T-] cells so clearly not what’s killing them. People with HIV/AIDS all have 30 to 60% reduced…glutathione… It’s a deficiency disease.

Sofia: [approx.. min.30] “So, let’s talk about AIDS as an energy deficiency disease… rather than being called Acquired Immune Deficiency, it should be called Acquired Energy Deficiency…”

Dr.Banks:  ”[Remember] that anti-oxidants provide energy, or provide electrons. Things that are oxidative take electrons. So glutathione has the sulf-hydryl group which is key to providing excess electrons in the cell. These excess electrons allow for all the normal functioning of the cell… normal cell division, normal use of the mitochondria…to allow one of the energy molecules, ATP, to develop… But the other important thing that happens in the mitochondria is that there are millions-per-second of…redox reactions [which] are simply reactions where an electron goes from one energy level to another energy level. When that happens, photons…create an electromagnetic wave and these waves are actually part of the energy of the cell, and part of the propagation of what allows the cell to keep itself together. So what happens if you…don’t have enough electrons to float that particular wave of energy in the cell…is you’re going to have…these reduced energy levels which do not allow the cell to function optimally… and that’s what’s going on with people said to have HIV.

…[Ebola in] Liberia, Sierra Leone and Guinea…just happens to break out where they just found [Aug.2013]..this huge cache of really expensive rocks [diamonds of 300+ karats]… They said Ebola was an RNA virus and the minute you hear [an epidemic] is RNA, you know immediately they’re not telling you the truth. Secondly, what is Ebola? –it’s a type of hemorrhagic fever, but there are many types…in tropical climates…and they’re trying to make people of afraid in temperate climates where tropical [diseases] don’t take hold, so that’s another nonsensical kind of thing, but we don’t really know what people are dying of… We know in this area the [World Health Organization] is testing all kinds of vaccines and drugs… but…the breakdown of the communities…and [civil] war [and dislocation] would cause diseases…and is no wonder…  March of 2014 was the first outbreak of Ebola and August 2013 [was] the announced diamond-mining discovery…

…”I do want to discuss…the RNA viruses. First there are two types of genetic material –DNA which is in our central genome and RNA…which has different [functions]. Now it turns out that it’s possible that the first type of genetic material on earth was RNA and…also almost 45% of the human genome is retro-transcribed…from RNA into DNA…and the term for it is ‘retrotransposons’ [or] DNA which is retro-transcribed from RNA… What it means is that this DNA, …what they used to call ‘junk DNA’…turns out that the more highly evolved a system is, the more so-called ‘junk DNA’ it has… But…this junk DNA actually has information –it’s messenger RNA and has some enzymatic functions… So as the cell functions and is communicating, it packages up these bits of genetic material [that] goes to different parts the cell or from cell to cell. If the cell is breaking down, then these bits of genetic material are transmitted out of the cell…   What they’ve been calling viruses, especially RNA viruses… most is not actually a virus but packaged pieces of normal genetic material…which are functioning the way they’re supposed to.

…”[In the past, from the 1920s], they believed that genes were stationary and didn’t move, transmitting information in one direction and [Barbara McClintock] said ‘no, genes are not stationary…they’re moving all the time’ …So you are evolving in terms of your environment because genes are always responding to…the environment…[and] move around almost like an alphabet making different words… We’re talking about a kind of consciousness at the energetic level… [It’s a dangerous idea] in a materialistic society because we don’t believe in consciousness at this level… The cell is completely quantum… so [knowledge of this] is going to break down the paradigm which is controlling science right now… [but] we’re moving into a whole new realm of how people think and behave.”

www.aboutthesky.com/podcasts/

  •                                                                   

*

“Disease [is] not an entity, but a fluctuating condition of the patient’s body, a battle between the substance of disease and the natural self-healing tendency of the body.” –Hippocrates

*

The authors of The Body Electric, Dr. Robert O. Becker and Gary Seldon, chose Hippocrates’s words to introduce Part 3 of their book, ‘Our Hidden Healing Energy.’  The concept of Hippocrates “fluctuating condition of the ..body” was iterated by Antoine Bechamp as ‘pleomorphism’ (or polymorphism) in ‘bioterrain’, a contradiction to his peers promoting Germ Theory, or monomorphism, posited by Robert Koch and Louis Pasteur. I learned originally of Bechamp from Dr. Banks in 2008 when I began working out the many biological problems in poliomyelitis as an environmental disease of radiation and chemical exposure. The Body Electric elevates these ‘bioterrain’ ideas into scientific proof of cellular abilities to ‘dedifferentiate and redifferentiate’ into the perfectly-needed cell types for healing and regeneration under the right cell-level electromagnetic conditions.

 

“I felt as though the temple curtain had been drawn aside without warning and I, a goggle-eyed stranger somehow mistaken for an initiate, had been ushered into a sanctuary to witness the mystery of mysteries. I saw a phantasmagoria, a living tapestry of forms jeweled in minute detail. They danced together like guests at a rowdy wedding. They changed their shapes. Within themselves they juggled geometrical shards like the fragments in a kaleidoscope. They sent forth extensions of themselves like the flares of suns. Yet all their activity was obviously interrelated; each being’s actions were in step with its neighbors’. They were like bees swarming: They obviously recognized each other and were communicating avidly, but it was impossible to know what they were saying. They enacted a pageant whose beauty awed me…   As the lights came back on, the auditorium seemed dull and unreal. I’d been watching various kinds of ordinary cells going about their daily business, as seen through a microscope and recorded by the latest time-lapse movie techniques. The filmmaker frankly admitted that neither he nor anyone else knew just what the cells were doing, or how and why they were doing it… The film was shown at a workshop on fracture healing sponsored by the National Academy of Sciences in 1965.” –pp135-136, The Body Electric, [Robert O. Becker MD] Becker and Seldon, 1985

“In the mid-1960s, solid-state devices were only beginning to hit the market, and one of the PN junction’s most interesting properties hadn’t yet been exploited. When you run a current through [a PN junction] in forward bias, some of its energy gets turned into light and emitted from the surface. In other words, electricity makes it glow. Nowadays various kinds of these PN junctions, called light-emitting diodes (LEDs), are everywhere… but then they were laboratory curios. We found that bone was an LED. Like many such materials, it required an outside source of light before an electric current would make it release its own light, and the light it emitted was at an infrared frequency invisible to us, but the effect was consistent and undeniable.” –p131, ibid.

“Semiconduction, [a]third kind of current, was a laboratory curiosity in the 1930s. Halfway between conductors and insulators, the semiconductors are inefficient in the sense that they can carry only small currents, but they can conduct their currents readily over long distances…  [Albert] Szent-Gyorgyi pointed out that the molecular structure of many parts of the cell was regular enough to support semiconduction… he conjectured that protein molecules, each having a sort of slot or way station for mobile electrons, might be joined together in long chains so that electrons could flow in a semiconducting current over long distances without losing energy…and passed along as information… I  [Becker] theorized that this system…regulated growth, healing, and perhaps other basic processes.” –p93, ibid.

“Ultimately we must relate all we learn about regeneration to a general system of communication among cells, for regrowth is only a special case of the cooperative cohesion that’s the essence of multicellular life…  The control system we’re seeking unites all levels of organization, from the idiosyncratic yet regular outline of the whole organism to the precisely engineered traceries of its microstructure. The DNA-RNA apparatus isn’t the whole secret of life, but a sort of computer program by which the real secret, the control system, expresses its pattern in terms of living cells.” –p182. Ibid. “The pituitary hormone prolactin, the same substance that stimulates milk flow in nursing mothers, seems to sensitize cells to electric current. Then the signal causes nearby cells to dedifferentiate…apparently by changing the way cell membranes pass calcium ions… Steve Smith then confirmed the importance of calcium by preventing dedifferentiation with a calcium-blocking compound, and restarting it with another substance that enhanced passage of calcium ions… Widespread…work on calcium-binding proteins, such as calmodulin, has made it fairly certain that electrical control of calcium movement through cell membranes directs the give-and-take among these proteins, which in turn supervises the cell’s entire genetic and metabolic industry… [The] available evidence suggests that the current flows through the perineural cells [in the myelin] rather than the neurons themselves… These are several types of cells that completely surround every nerve cell, enclosing all the peripheral fibers in a sheath and composing 90 percent of the brain… Not all cells can respond, however…” –p183—“…[but] we reflect on the stakes: understanding regeneration well enough to restore it to ourselves. Certainly skin is electrically active. It’s piezoelectric and pyroelectric (turning heat into electricity) as well as a transporter of ions in wet animals. In the last two decades [1965-1985] nearly all tissues have been proven to produce or carry various kinds of electrical charge. Skin may play an as-yet-unknown role in regeneration… We can infer two things about the control [system]…in relation to the whole organism [;] the guidance can’t be purely local, but must come from a system that likewise pervades the whole body. Furthermore, there are no dedifferentiated cells left over when the work is done; there are just enough and no more… A large body of earlier work has shown that redifferentiation instructions are passed along a tissue arc whose main element is the circuit already established between nerves and epidermis” –p185—“The direction (polarity) plus the magnitude and force (amperage and voltage) of current could serve as a vector system… The electric field surrounding continuously charged cells…would provide a third coordinate…[and] a magnetic field…possibly adding a fourth dimension… Together these values might suffice to pinpoint any cell in the body. The electric and magnetic fields, varying as the current varies with the animal’s state of consciousness and health, could move charged molecules wherever they were needed for control of growth or other processes.” –p186—“As one who has performed too many amputations in his time, I find the prospect of being able to give a patient the real thing instead of a prosthesis tremendously exciting. There’s a good chance…such techniques could even rectify genetic birth defects. Since no one has yet achieved full regeneration…these dreams won’t come true overnight. They aren’t chimerical, however. The remaining problems could probably be solved…[by] basic research. Meanwhile, human capacities for repair of certain tissues are greater than most people realize, and there are already promising ways…” –p187, The Body Electric, 1985

*

*

*

*

 

 

May 12, 2020

COVID: DNA Dragnet

  •                                                                

 

COVID DNA Dragnet: A brink-of-reality scenario based on what’s already happening, such as contact-tracing, “rogue DNA databases,” and ideas in circulation posing as alternatives to vaccines and chip implants –at least temporarily. DNA dragnets already occur in policing. It doesn’t take a pandemic for this to realize, but the advantage of  timing and cooperation is obvious. You would take a test, wouldn’t you—to be cleared for work or travel? As I see it right now, our collective loss of liberties puts us one step away from national COVID dragnets to get everyone in the DNA database networks in the name of Public Health. It can happen with or without your knowledge and consent through the surreptitious collection and storage of DNA as it is widely practiced by law enforcement in the U.S.  Police methods regarding DNA have been upheld and re-sanctioned by the Supreme Court. [Maryland, 2012, Chief Justice John G. Roberts issued an order allowing police to continue collecting DNA samples]. Collection methods vary from trash-picking to free dinners, to coerced ‘voluntary’ clearing-of-suspicion and mandatory swabbing on arrest. Methods vary as much as the departments that deploy them. Preface this with facts of “Fabricating DNA Evidence” from digital bioinformatics systems [posted here Aug.2009] and the direst possibilities just start popping –but I won’t imagine those now. Just the facts.  On-the-spot COVID tests are coming –police already have spot tests for DNA—useable at building entrances or any convenient or  especially inconvenient location. The race is on for getting new, fast, portable and cheap CoV tests to market, such as a CoV ‘breath-alyzer’ from the UK.   So– a race for ‘better’ tests? …for the purpose of X@#B&5???? Is the dragnet ON?

  •               Checkpoint COVID*

Police methods: ”Rogue DNA databases” are a subject of special scrutiny by law professor Erin E. Murphy in her 2015 book “Inside the Cell, The Dark Side of Forensic DNA” which reviews issues of disrepute in DNA collection in post 9/11 America. The ‘rogues’ operate outside the law, however they are legal and actually contribute information to larger and better governed databases. The collected DNA, overall and everywhere, is subject to the “doctrine of abandonment”, the same criterion used in medical research. Collection-keeping for police uses are, in theory and practice, almost identical to medical uses, and therefore ‘one step away’ which is the ‘legal authority’ that comes with a national emergency waiver.

*

Telephone-based contact tracing: “The CDC defines a close contact as someone within 6 feet of an infected person for at least 15 minutes”…but “Once you see a big escalation in cases, contact tracing absolutely cannot work,” Osterholm said. “You will be having contacts by the thousands and thousands and thousands. It’s just not going to work.” https://www.usatoday.com/story/news/health/2020/05/13/coronavirus-states-scramble-hire-covid-19-contact-tracers/3088014001/

“Contact tracing apps may complement human contact tracing and add efficiency, but they don’t replace all the things you can do training armies of contact tracers”  …“If there is a three-day lag between the test and results, depending on where that person goes, hundreds of people could be exposed — versus if a person is tested and gets the results 15 minutes later, they can begin taking precautions immediately,”…   https://health4everyday.com/2020/05/lost-your-job-consider-becoming-a-contact-tracer-cbs-news/

New contact tracers in the U.S., for now, are entry level data processors, building a database for Public Health. Tracers may also soon adopt police search methods, being done elsewhere, from identities drawn from all possible surveillance sources; security cams, cell phones, credit and debit cards, etc.This goes sideways in all directions really fast.

*

When pandemic COVID policing and law enforcement merge, Public Health has a vast and instant connectivity for contact tracing:

“Across the country, police departments employ sneak DNA sampling methods without judicial intervention of any kind, and obtain biological material… As the head of one government lab said, ‘When you’ve licked a stamp on your tax return you’ve sent the government a DNA sample.’  …The true scope and extent of sneak sampling practices is not known… [but] in many cases police simply keep surreptitiously gathered samples, adding those DNA profiles to offline or rogue databases that can be searched without restriction. Thus both the data and the databases are shrouded in secrecy…  Oddly enough, the law essentially encourages police to act in the sneakiest ways possible, by according the least oversight and protection to DNA samples collected on the sly… [C]urrent law places no limits on the use of the sample. Police can keep those samples indefinitely, test them as they see fit, and store them in their local database for use in future cases—and all without ever notifying any person that DNA has been sampled. The courts that have addressed sneak sampling have almost universally endorsed it as acceptable under the Fourth Amendment…  [A comment made after a failed case argument challenging the sneak techniques] reflects, the attitude of courts toward surreptitious sampling appears to be that the precepts of ‘abandonment’ and ‘misplaced trust’ ought to apply down to the molecular level… [pp170-172]  …

“[If a person] voluntarily gave over his sample, the police were entitled to use it in any way they saw fit…  Rogue databases…have sprung up around the country…[which] can be as simple as Excel spreadsheets or as sophisticated as networked terminals that mimic the national database, CODIS… The New York City database came under fire in 2005 when…litigation revealed that the city maintained a ‘Linkage Database’ that included samples not just from arrestees and suspects, but from crime scene bystanders and Good Samaritan contributors. The lab admitted it had ‘no policy’, procedure or rules for purging profiles from the database, and that there were no restrictions on the database’s permissible use.[p183] Rogue databases also offer the opportunity to store genetic information beyond that contained in the customary national DNA profile… New Mexico maintains a rogue DNA database…shown to be effective at predicting surnames, particularly rare surnames. [p184] The attractiveness of a database that operates outside the law has led genetic entrepreneurs to start offering software that links them all together, creating a…shadowbase…of the federal database. [p185] The goal was to circumvent CODIS, which precluded uploading of samples from persons not charged with crimes or who gave DNA for elimination purposes only. [p186]…To be sure, solving crime is good. But creating a nation of suspects is not.” [p172]

Inside The Cell, by Erin E. Murphy, 2015.

*

*

*

May 10, 2020

Accelerating Biology

*                                                                                   

 

Taking control of your body: Lessons from a cell biologist

“While the Human Genome Project was making headlines, a group of scientists were inaugurating a new, revolutionary field in biology called epigenetics. The science of epigenetics, which literally means ‘control above genetics,’ profoundly changes our understanding of how life is controlled… Genes are not destiny! Environmental influences, including nutrition, stress and emotions, can modify those genes without changing their basic blueprint. And those modifications, epigeneticists have discovered, can be passed on to future generations as surely as DNA blueprints are passed on via the Double Helix.” –p67, Biology of Belief, by Bruce H. Lipton, PhD, 2005

“You may consider yourself an individual, but as a cell biologist I can tell you that you are in truth a cooperative community of approximately 50 trillion single-cell citizens. Almost all of the cells that make up your body are amoeba-like, individual organisms that have evolved a cooperative strategy for their mutual survival. [p27]… I also [make] it clear to my students that each cell is an intelligent being that can survive on its own, as scientists demonstrate when they remove cells from the body and grow them in a culture. [p37] I knew intuitively when I was a child, these smart cells are imbued with intent and purpose; they actively seek environments that support their survival while simultaneously avoiding toxic or hostile ones. Like humans, single cells analyze thousands of stimuli from the microenvironment they inhabit… [and] select appropriate behavioral responses to ensure their survival. Single cells are also capable of learning through these environmental experiences and are able to create cellular memories which they pass on to their offspring.” [p38]

The evolutionary push for ever-bigger communities is simply a reflection of the biological imperative to survive. The more awareness an organism has of its environment, the better its chances for survival. When cells band together [i.e. into higher organisms] they increase their awareness exponentially… A process of cytological specialization enables the cells to form the specific tissues and organs of the body. Over time, this pattern of differentiation, i.e. the distribution of the workload among the members of the community, became embedded in the genes of every cell… significantly increasing the organism’s efficiency and its ability to survive… The efficiency it offered enabled more cells to live on less… The amount of energy conserved…contributes to both an increased survival advantage and a better quality of life. [p40]

But today’s understanding of cooperation in nature goes much deeper than the easily observable ones. ‘Biologists are becoming increasingly aware that animals have coevolved, and continue to coexist with diverse assemblages of microorganisms that are required for normal health and development,’ according to..an article in Science [Ruby, et.al. 2004] The study of these relationships is now a rapidly growing field called ‘Systems Biology.’ …Living organisms, it turns out, actually integrate their cellular communities by sharing their genes… Now scientists realize that genes are shared not only among the individual members of a species, but also among members of different species. The sharing of genetic information via gene transfer speeds up evolution since organisms can acquire ‘learned’ experience from other organisms. [p44] This sharing of information is…nature’s method of enhancing the survival of the biosphere… [and] Now that we are aware of this inter-and-intra-species gene transfer mechanism, the dangers of genetic engineering become apparent. [p45]

“ In reality, the idea that genes control biology is a supposition, which has never been proven and in fact has been undermined by the latest scientific research. Genetic control…has become a metaphor in our society. We want to believe that genetic engineers are the new medical magicians who can cure diseases and while they’re at it create more Einsteins and Mozarts as well. But metaphor does not equate with scientific truth. [H.F.] Nijhout (1992) summarizes the truth: ‘When a gene product is needed, a signal from its environment, not an emergent property of the gene itself, activates expression of that gene.’ In other words, when it comes to genetic control, ‘It’s the environment…’ [p52]…[and] it is the changing of the proteins’ electromagnetic charges that is responsible for their behavior-generating movement, not DNA. [p60]. Geneticists experienced a… shock when, contrary to their expectations of [finding] over 120,000 [human] genes, they found that the entire human genome consists of approximately 25,000 genes. More than eighty percent of the presumed and required DNA does not exist!… The one-gene, one-protein concept was a fundamental tenet of genetic determinism… No longer is it possible to believe that genetic engineers can with relative ease fix all our biological dilemnas. There are simply not enough genes to account for the complexity of human life or human disease. [p63]

“In the chromosome, the DNA forms the core and the [regulatory] proteins cover the DNA like a sleeve. When the genes are covered, their information cannot be ‘read.’  …How do you get that sleeve off? You need an environmental signal to spur the ‘sleeve’ protein to change shape, i.e. detach from the DNA’s double helix, allowing the gene to be read [and copied by the cell]… As a result, the activity of the gene is ‘controlled’ by the presence or absence of the ensleeving proteins which are in turn controlled by environmental signals. [p68] The story of epigenetic control is the story of how environmental signals control the activity of genes… The revised scheme of information flow…in biology starts with an environmental signal, then goes to a regulatory protein and only then goes to DNA, RNA, and the end result, a protein. The science of epigenetics has also made clear that there are two mechanisms by which organisms pass on hereditary information… Studies of protein synthesis reveal that epigenetic [tuning]‘dials’ can create 2,000 or more variations of proteins from the same gene blueprint. [p69]

“We now know that the environmentally influenced fine-tuning…can be passed from generation to generation. A landmark Duke University study published in the August 2003 issue of Molecular and Cellular Biology found that an enriched environment can even override genetic mutations in mice. In the study, scientists looked at the effect of dietary supplements on pregnant mice with the abnormal ‘agouti’ gene. Agouti mice have yellow coats and are extremely obese, which predisposes them to cardiovascular disease, diabetes and cancer. In the experiment, one group of yellow, obese, agouti mothers received methyl-group rich supplements available in health food stores: folic acid, vitamin B12, betaine and choline. Methyl-rich supplements were chosen because a number of studies have shown that the methyl chemical group is involved with epigenetic modifications. When methyl groups attach to a gene’s DNA, it changes the binding characteristics of regulatory chromosomal proteins. If the proteins bind too tightly to the gene, the protein cannot be removed and the gene cannot be read. Methylating DNA can silence or modify gene activity… The mothers who got the methyl group supplements produced standard, lean, brown mice, even though their offspring had the same agouti gene as their mothers. The agouti mothers who didn’t get the supplements produced yellow pups…which ate much more [and] wound up weighing almost twice as much as their… counterparts… [and were] diabetic while its genetically identical counterpart is healthy. [p72]

  •                                                                            

 

*

“Living organisms are distinguished from non-living entities by the fact that they move: they are animated. The energy driving their movements is harnessed to do the ‘work’…such as respiration, digestion, and muscle contraction. To understand the nature of life one must first understand how protein ‘machines’ are empowered to move. The final shape, or conformation (the technical term used by biologists), of a protein molecule reflects a balanced state among its electromagnetic charges. However, if the proteins positive and negative charges are altered, the protein backbone will dynamically twist and adjust itself to accommodate the new distribution of charges. [Like charges, as in two negative terminals, will twist apart]. The distribution of electromagnetic charge within a protein can be selectively altered by a number of processes including: the binding of other molecules or chemical groups such as hormones; the enzymatic removal or addition of charged ions; or interference from electromagnetic fields such as those emanating from cell phones. [p56] [It] is the changing of the proteins’ electromagnetic charges that is responsible for their behavior-generating movement, not DNA. [p60]

“[My] nominee for the true brain that controls cellular life [is] the membrane… I refer to it in my lectures as the magical mem-Brain… The true secret of life lies in understanding the elegantly simple biological mechanisms of the magical membrane –the mechanisms by which your body translates environmental signals into behavior… Cell biologists gained insight into the amazing abilities of the cell membrane by studying the most primitive organisms on this planet, the prokaryotes… which include bacteria and other microbes [that] consist only of a cell membrane that envelops a droplet of soupy cytoplasm… A bacterium carries out the basic physiologic processes of life like more complicated cells… They can sense where there is food… they can recognize toxins and predators and purposely employ escape maneuvers to save their lives. In other words, prokaryotes display intelligence! [p76]

“It is important for the cell to allow molecules to break through the [membrane] barrier..[for] life-sustaining food…[and] information… [The] membrane becomes a vital and ingenious mechanism enabling selected nutrients to penetrate the interior of the cell… Phospholipids, one of the two major chemical components of the membrane…are composed of both polar and non-polar molecules…. All the molecules in our universe can be divided into non-polar and polar categories based on the type of chemical bonds that hold their atoms together. The bonds among [electrically charged] polar molecules…behave like magnets, attracting or repelling other charged molecules. Polar molecules include water and things that dissolve in water [hydrophiles]. Non-polar molecules include oil and substances that dissolve in oil [hydrophobes]… The phosphate portion of the molecule is motivated to seek water, while its lipid portion…seeks stability by dissolving in oil. [p80]…Because the [oil] portion of the membrane is non-polar, it does not let positively or negatively charged atoms or molecules pass through it. In effect, this lipid core is an electrical insulator…[but..]Most of the cell’s nutrients consist of charged polar molecules that would not be able to get past the formidable non-polar lipid barrier. Neither could the cell excrete its polarized waste products.  [Embedded membrane proteins, called]Integral Membrane Proteins (IMPs),…are composed of a linear backbone assembled from linked amino acids…[where] some are water-loving polar molecules and some are hydrophobic, non-polar molecules…that weaves itself in and out of the [membrane layer]. There are lots of IMPs with lots of different names, but they can be subdivided into two functional classes: receptor proteins and effector proteins… Receptors function as molecular ‘nano-antennas’ tuned to respond to specific environmental signals. Some receptors extend inward…to monitor the internal milieu of the cell. Other[s]…monitor external signals… Receptor ‘antennas’ can also read vibrational energy fields such as light, sound, and radio frequencies. The antennas on these ‘energy’ receptors vibrate like tuning forks. If an energy vibration in the environment resonates with a receptor’s antenna, it will alter the protein’s charge, causing the receptor to change shape.[p83]. [And] because receptors can read energy fields, the notion that only physical molecules can impact cell physiology is outmoded. Biological behavior can be controlled by invisible forces, including thought, as well as it can be controlled by physical molecules like penicillin, a fact that provides the scientific underpinning for pharmaceutical-free energy medicine. [p84]

“When you destroy its membrane, the cell dies just as you would if your brain were removed. Even if you leave the membrane intact, destroying only its receptor proteins, which can easily be done with digestive enzymes in the lab, the cell becomes ‘brain-dead.’ It is comatose because it no longer receives the environmental signals necessary for the operation of the cell. The cell also becomes comatose when the receptor proteins are left intact and its effector proteins are immobilized. To exhibit ‘intelligent’ behavior, cells need a functioning membrane with both receptor (awareness) and effector (action) proteins. These protein complexes are the fundamental units of cellular intelligence. Technically they may be referred to as units of ‘perception.’ …At the cellular level, the story of evolution is largely the story of maximizing the number of basic units of ‘intelligence,’ the membrane’s receptor/effector  proteins. Cells  become smarter by utilizing their outer membrane surface more efficiently and by expanding the surface area..so that more IMPs could be packed in… the eukaryote is thousands of times bigger than the prokaryote resulting in a tremendous increase in membrane surface area… The end result is more awareness, which translates to greater survivability. [p88]

“In 1985…I was reviewing the mechanics of the membrane trying to get a grasp of how it worked as an information processing system. That is when I experienced a moment of insight…[starting] first with the lollipop-like phospholipid molecules…arranged in the membrane like regimented soldiers on parade in perfect alignment. By definition, a structure whose molecules are arranged in regular, repeated pattern is defined as a crystal. There are two fundamental types of crystals. The crystals that most people are familiar with are hard and resilient minerals… The second kind of crystal has a more fluid structure…Familiar examples of liquid crystals include digital watch faces and laptop computer screens. To better understand the nature of a liquid crystal, let’s go back to those soldiers on parade. When the marching soldiers turn a corner, they maintain their regimented structure… They’re behaving like a flowing liquid, yet they do not lose their crystalline organization. The phospholipid molecules of the membrane behave in a similar fashion. Their fluid crystalline organization allows the membrane to dynamically alter its shape while maintaining its integrity…So in defining this character of the membrane I wrote: ‘The membrane is a liquid crystal.’ Then I started thinking about…a membrane with just phospholipids…[being] a non-conductor. However, when you add IMPs [integral membrane proteins], you realize that the membrane conducts some things across while keeping others out. So I [wrote]…’The membrane is a semiconductor.’  Lastly, I wanted to include…the two most common kinds of IMPs. These are the receptors and a class of effectors called channels because they [change shape and] provide the all-important means for the cell to let in nutrients and let out waste matter. I was about to write..’recptors and channels’ when I realized that a synonym for receptor is the word gate. So instead I completed my description by writing: ‘The membrane contains gates and channels.’ [p90] What hit me right away was… my new computer [with] a copy of a bright red book called ‘Understanding Your Microprocessor’… I grabbed the book and found..a definition of a computer chip that read: ‘A chip is a crystal semiconductor with gates and channels.’…I spent several more intense seconds comparing… biomembranes with silicon semiconductors… when I realized that the identical nature of their definitions… Twelve years later an Australian research consortium…published an article in Nature, which confirmed my hypothesis that the cell membrane is a homologue of a computer chip. [B.A.Cornell, et al. 1997] Cornell and associates successfully turned a biological cell membrane into a digital-readout computer chip. [p91] …The fact that the cell membrane and a computer chip are homologues means that it is both appropriate and instructive to better fathom the workings of a cell by comparing it to a personal computer. The first big-deal insight that comes from such an exercise is that computers and cells are programmable…[and] the programmer lies outside the computer/cell…[It was with a jolt that I realized that the gene-coding nucleus does not program the cell. [p92]

  •                                                           

*

“[B]ecause of their Newtonian, materialistic bias, conventional researchers have completely ignored the role that energy plays in health and disease. [p102] Biomedical scientists have been particularly confounded because they do not recognize the massive complexity of the intercommunication among the physical parts and the energy fields that make up the whole… Cellular contsituents are woven into a complex web of crosstalk, feeback and feedforward communication loops. A biological dysfunction may arise from a miscommunication along any of the routes of information flow… Once I realized the nature of the complex interactions between matter and energy, I knew that a reductionist, linear (A>B>C>D>E) approach could not even come close to giving us accurate understanding of disease. [p103] When you change the parameters of a protein at one point in such a complex pathway, you inevitably alter the parameters of other proteins at innumerable points within the entangled networks. [p104] When a drug is introduced into the body to treat a malfunction in one protein, that drug inevitably interacts with at least one and possibly many other proteins… [There is] the fact that biological systems are redundant. The same signals or protein molecules [p105] may be simultaneously used in different organs and tissues where they provide for completely different behavioral functions. For example, when a drug is prescribed to correct a dysfunction in a signaling pathway of the heart, that drug is delivered by the blood to the entire body. This ‘cardiac’ medicine can unintentionally disturb the function of the nervous system if the brain also uses components of the targeted signaling pathway…. Multicellular organisms survive with far fewer genes than scientists once thought because the same gene products (protein) are used for a variety of functions. [p106]

“ Hundreds upon hundreds of scientific studies over the last fifty years have consistently revealed that ‘invisible forces’ of the electromagnetic spectrum profoundly impact every facet of biological regulation… [E]nergetic signaling mechanisms such as electromagnetic frequencies are a hundred times more efficient in relaying environmental information than physical signals such as hormones, neurotransmitters, growth factors, etc.[p111] We know that living organisms must receive and interpret environmental signals in order to stay alive. In fact, survival is directly related to the speed and efficiency of signal transfer. The speed of electromagnetic energy signals is 186,000 miles per second, while the speed of a diffusible chemical is considerably less than 1 centimeter per second. Energy signals are 100 times more efficient and infinitely faster than physical chemical signaling. What kind of signaling would your trillion-celled community prefer? Do the math!

…”all organisms, including humans, communicate and read their environment by evaluating energy fields. [p120] The research I discussed…found that energy is a more efficient means of effecting matter than chemicals. [p125] Candace Pert was studying the human brain… In Molecules of Emotion,  Pert revealed how her study of information-processing receptors on nerve cell membranes led her to discover that the same ‘neural’ receptors were present on most, if not all, of the body’s cells. Her elegant experiments established that the ‘mind’ was not focused in the head, but was distributed via signal molecules to the whole body. [p132]

“Humans and a number of other higher mammals have evolved a specialized region of the brain associated with thinking, planning, and decision-making called the prefrontal cortex. This portion of the fore-brain is apparently the seat of the ‘self-conscious’ mind processing…it is a newly evolved ‘sense organ’ that observes our own behavior and emotions. [It] also has access to most of the data stored in our long-term memory bank [including cells]… Endowed with the ability to be self-reflective, the self-conscious mind is extremely powerful… We can actively choose how to respond to most envorinmental signals and whether we even want to respond at all. The conscious mind’s capacity to override the sunconscious mind’s preprogrammed behaviors is the foundation of free will. [p134]

“I was excited by my experiments because I believed that they revealed on the single-cell level a truth for multicellular organisms—that the mind (i.e. acting via the central nervous system’s adrenaline) overrides the body (acting via the local histamine signal). I wanted to spell out the implications…in my research paper, but my colleagues almost died from apoplexy at the notion of [a] body-mind connection into a paper about cell biology… because the mind is not an acceptable biological concept. Bioscientists are conventional Newtonians –if it isn’t matter…it doesn’t count. The ‘mind’ is a non-localized energy and therefore is not relevant to materialistic biology. [p137] The placebo effect should be the subject of major, funded research efforts. If medical researchers could figure out how to leverage the placebo effect, they would hand doctors an efficient, energy-based, side effect-free tool to treat disease… The fact that most doctors are not trained to consider the impact of the placebo effect is ironic because some historians make a strong case that the history of medicine is largely the history of the placebo effect… [p138] [including] ‘fake’ surgery. [p139]

*

*

“In multicellular organisms, growth/protection behaviors are controlled by the nervous system. It is the nervous system’s job to monitor environmental signals, interpret them, and organize appropriate behavioral responses… the nervous system acts like the government in organizing the activities of its cellular citizens… The body is actually endowed with two separate protection systems, each vital to the maintenance of life. The first…mobilizes protection against external threats. It is called the HPA axis which stands for the Hypothalamus-Pituitary-Adrenal Axis. [p147] When there are no threats, the HPA axis is inactive and growth [cell renewal, respiration, digestion, etc.] flourishes… Once the adrenal alarm is sounded… [the] visceral organs stop doing their life-sustaining work of digestion, absorption, excretion and…production of the body’s energy reserves. Hence the stress response inhibits growth processes and further compromises the body’s survival by interfering with the generation of vital energy reserves. [p148]

“ The body’s second protection system is the immune system which protects us from threats originating under the skin such as those caused by bacteria and viruses… it can consume much of the body’s energy supply. [p149] The HPA system is a brilliant mechanism for handling acute stresses. However…not designed to be continuously activated.[p151] The HPA axis’ effect on the cellular community mirrors the effect of stress on a human population. [p153] [It shifts] the members of the community from a state of growth to a state of protection. [p154] …[S]tress hormones are so effective at curtailing immune system function that doctors provide them to recipients of transplants so that their immune systems wouldn’t reject the foreign tissues…. Activating the HPA axis also interferes with our ability to think clearly… Adrenal stress hormones constrict the blood vessels in the forebrain…[and] repress activity in the…prefrontal cortex…the center of conscious volitional activity… and reasoning. [p150]

“Inhibiting growth processes [which includes natural immunity] is also debilitating in that growth…is required to produce energy. Consequently, a sustained protection response inhibits the creation of life-sustaining energy. The longer you stay in protection, the more you compromise your growth…To fully thrive, we must not only eliminate the stressors but also actively seek joyful, loving, fulfilling lives that stimulate growth processes.” [p147] Biology of Belief, by Bruce H. Lipton, PhD

………………………………give you some love…………………………..

*

*

*

 

May 5, 2020

COVID: Going to the Dogs

  •                                                    

*

Pet dogs, pet cats, lions and tigers at the zoo—all testing positive for SARS-CoV-2. Wuhan reported that 15% of the housecats tested were positive for COVID-19 antibodies, but no case of illness was reported in Chinese kitty-cats. The first COVID-19 dog case in China, a 17-yr-old Pomeranian, was held in quarantine longer than its CoV-positive owner, and died 2 days after being released, at which time it was negative, never having shown any symptoms. The second COVID-positive dog in China, a pet German shepherd, showed no signs or symptoms and was still remanded to quarantine. The United States, however, is reporting illness; the first U.S. dog case is a pug named Winston who developed a cough –and he’s doing fine now. His owners are a COVID-positive family of three with other pets, and part of the medical community in North Carolina.

*

Five tigers and three lions at the Bronx Zoo are all presumed to have contracted COVID-19 from a single human handler. But this is where the story gets interesting because zoo animals have been subject to long-term observation with scientific analyses –a situation reviewed in my current ‘go-to’ resource for electrosensitivity diseases—The Invisible Rainbow.  The startling awareness at the moment is that Arthur Firstenberg’s 2017 book is a virtual guide to the COVID pandemic.

*

Hang on, because this is an intricate endeavor. I don’t want to bombard you with technicalities as I go through a COVID series of posts. The epidemiology and ‘underlying condition’ causes are essential to know and what it’s all about, including the social, cultural, unconstitutional and likewise. The genetic, biomedical, and biotechnology information about coronavirus and its sequelae correlates in full.  At the same time, on the technical front, I’m just a few steps ahead of my posts in this maze – a-maze-ing. Yeah. That said, Mr. Firstenberg, no doubt a principled 4-year medical student, vegetarian, and activist who dedicated his book to a beloved friend beset with porphyria, has filled a void left derelict by our public health policy professionals. I bought Firstenberg’s book in the first place to help me fill out the composited health effects in my research on influenza and polio, diseases of radiation exposure –he doesn’t at all deal with polio, nuclear industry, or radioactive materials and fallout. Rather sticking scrupulously to electrification and microwaves as we know them, The Invisible Rainbow drops you right in the memory hole.  A memory hole, a very old expression coined from the native Americans, is not where information goes to die of neglect, but where it goes to be found and cherished.

  •                                                             *

*It’s all happening at the Zoo.*

In chapter 11, “Irritable Heart”, Arthur Firstenberg writes, “Whatever environmental factor was affecting human beings in America during the 1930s and 1940s was also affecting all the animals in the Philadelphia Zoo. The Laboratory of Comparative Pathology was a unique facility founded at the zoo in 1901. And from 1916 to 1964 [they] kept complete records of autopsies performed on over thirteen thousand animals that had died in the zoo. During this period, arteriosclerosis increased an astonishing ten-to-twenty-fold among all species of mammals and birds. In 1923, [Herbert] Fox had written that such lesions were ‘exceedingly rare,’ occurring in less than two percent of animals as a…finding at autopsy. The incidence rose rapidly during the 1930s, and by the 1950s arteriosclerosis was not only occurring in young animals, but was often the cause of their death… Coronary heart disease appeared even more suddenly. In fact. Before 1945 the disease did not exist at the zoo. And the first heart attacks ever recorded in zoo animals occurred ten years later, in 1955… [By] 1963, over 90 percent of all mammals and 72 percent of all birds that died in the zoo had coronary disease, while 24 percent of the mammals and 10 percent of the birds had had heart attacks. And the majority of heart attacks were occurring in young animals in the first half of their expected life spans… Diet had nothing to do with these changes… [D]iets were the same at all times between 1935 and 1964… [T]housands of miles away, researchers were finding arteriosclerosis in 22 percent of the animals in the London Zoo in 1960, and a similar number in the Zoo of Antwerp Belgium in 1962. The element that increased most spectacularly in the environment during the 1950s when coronary disease was exploding among humans and animals was radio frequency (RF) radiation.” –pp171-172, The Invisible Rainbow

“Trained as a biologist, Allan H. Frey became interested in microwave research in 1960 by following his curiosity… He left his job at General Electric and began doing full-time research into the biological effects of microwave radiation. In 1961, he published his first paper on ‘microwave hearing’ [which Frey himself could hear]… He spent the next two decades experimenting on animals…to clarify [RF] effects on the auditory system, the eyes, the brain, the nervous system, and the heart. He discovered the blood-brain barrier effect, an alarming damage to the protective shield that keeps bacteria, viruses, and toxic chemicals out of the brain –damage that occurs at levels of radiation that are much lower than what is emitted by cells phones today… The effects Frey demonstrated occur because the heart is an electrical organ and microwave pulses interfere with the heart’s pacemaker. But in addition to these direct effects, there is a more basic problem: microwave radiation, and electricity in general, starves the heart of oxygen because of effects at the cellular level.” –pp176-177, ibid. “The fundamental defect…is that although enough oxygen and nutrients reach the cells, the mitochondria –the powerhouse of the cells—cannot efficiently use that oxygen and those nutrients, and not enough energy is produced to satisfy the requirements of heart, brain, muscles, and organs. This effectively starves the entire body, including the heart, of oxygen, and can eventually damage the heart.” –p187, ibid.

–oxidative stress, a cause in AIDS we learn from Dr. Nancy Banks’ book AIDS, Opium, Diamonds, and Empire

*

Because of homology across species and ‘receptors,’ we sink or swim, crawl, leap and fly together. One Medicine. One World. “One level of Health for all…” (NIH lobbyist Florence S. Mahoney)

  •                                                                    *

 

The Chapel Hill, North Carolina family, owners of Winston the pug, are enrolled in a special study called MESSI –for Molecular and Epidemiological Study of Suspected Infection –messy, got it?—a DoD-funded project headed by Dr. Christopher W. Woods. “Woods is a principal investigator [PI] in a $3.8 million DARPA project to help develop genetic test[s] for exposure to weapons of mass destruction.” https://precisionmedicine.duke.edu/MESSI-study

 “His research focuses on the development of novel diagnostic approaches to infectious disease and the potential for interspecies transmission of pathogens. His genomic approach to harnessing the host response for diagnosis of infectious diseases has been called a paradigm shift in the field. Dr. Woods is a partner in the Southeastern Center for Emerging Biological Threats, core PI of the Southeastern Research Center for Excellence on Emerging Infections and Biodefense, and a leader in the NIH-funded Vaccine and Therapeutics Evaluation Unit at Duke.” https://globalhealth.duke.edu/people/woods-chris

If you are COVID positive, have a pet, and would like to enroll in a study, there are multiple “One Health” initiatives underway. University of Washington One Health Research asks not to include birds or reptiles, and if you have one, no pangolins.

*pangolin*

“Pangolin CoV and one type of bat CoV are likely to be able to use both human and other animal ACE2 to gain entry into the host cells. Moreover, the SARS-CoV-2 virus can bind to multiple animal ACE2s even if the furin cleavage site is deleted. Thus, many of these wild animals can be natural hosts or intermediate hosts for the novel coronavirus and its progenitor.” https://www.news-medical.net/news/20200420/ACE2-receptor-expression-by-SARS-CoV-2-across-different-animal-species.aspx

**********************************************************

*

May 3, 2020

COVID: Accelerating Electrosensitivity

*                                                             *

*

 

I don’t have a TV but I do listen to the radio –NPR precisely– for 2 or 3 hours in the day. It’s the only station I can reliably tune-in, and ever since Sandy Hook when I learned that NPR was the principal partner in that media event, I listen. It’s the EAS (Emergency Alert System) mouthpiece of The Agenda. Our national radio theater is obsessed with the ‘changing world’ scenario of COVID. Radio guest Richard N. Haass, consultant to Bush I, Bush II, and president of the CFR, told the audience that COVID is “accelerating  history” (globalization) and is “not a choice.” Indeed. The “Rockefeller Model” of bottom-up transition is to “make the peaks higher,” or in this case the spikes, as we slide ahead — by the head–  into the Internet of Things.

*

Here’s a biology and health lesson from The Invisible Rainbow by Arthur Firstenberg. Everything below is quotation except [bracketts]:

“Porphyrins are light sensitive pigments that play pivotal roles in the economy of both plants and animals…  In plants a porphyrin bound to magnesium is the pigment called chlorophyll…responsible for photosynthesis. In animals an almost identical molecule bound to iron is the pigment called heme, the essential part of hemoglobin that makes blood red and enables it to carry oxygen… Heme is also the central component of…enzymes…in every cell of every plant, animal and bacterium, that transport electrons from nutrients to oxygen so that our cells can extract energy. And heme is the main component of the cytochrome P-450 enzymes in our liver that detoxify environmental chemicals for us by oxidizing them.

“In other words, porphyrins are the very special molecules that interface between oxygen and life. They are responsible for the creation, maintenance, and recycling of all the oxygen in our atmosphere… The high reactivity of these molecules, which makes them the transformers of energy and their affinity for heavy metals, also makes them toxic when they accumulate in excess in the body, as happens in the disease called porphyria… (p136)   The enzymes of the heme pathway are among the most sensitive elements of the body to environmental toxins. (p137).  [In] a world in which toxic chemicals are inescapable, the porphyrin pathway is to some degree always stressed, and only those with high enough enzyme levels tolerate the pollution.

…[In] the early 1990s…Dr. William E. Morton, professor of occupational and environmental medicine at Oregon Health Sciences University…proposed that the controversial disease called multiple chemical sensitivity (MCS) was in most cases identical with one or more forms of porphyria. And when he began testing his MCS patients he found that, indeed, 90 percent of them were deficient in one or more porphyrin enzymes… Morton also found that most people with electrical sensitivity had porphyrin enzyme deficiencies, and that electrical and chemical sensitivities appeared to be manifestations of the same disease. Porphyria, Morton showed, is not the extremely rare [and misdiagnosed] illness it is currently thought to be, but has to affect at least five to ten percent of the world’s population. (p138).

“Porphyrins are central to our story not only because of a disease named porphyria, which affects a few percent of the population [perhaps 40-75 million people] but because of the part porphyrins play in the modern epidemics of heart disease, cancer, and diabetes which affect half the world, and because their very existence is a reminder of the role of electricity in life itself… (p139-140)

“Piezoelectricity, a property of crystals that makes them useful in electronic products that transforms mechanical stress into electrical voltages and vice versa, has been found in cellulose, collagen, horn, bone, wool, wood, tendon, blood vessel walls, muscle, nerve, fibrin, DNA, and every type of protein examined. In other words –something most biologists have been denying for two centuries—electricity is essential to biology… It was Otto Lehmann, already in 1908, who noticing the close resemblance between the shapes of known liquid crystals and many biological structures, proposed that the very basis of life was the liquid crystalline state. Liquid crystals, like organisms, had the ability to grow..; to heal wounds; to consume other substances or other crystals; to be poisoned; to form membranes; spheres, rods filaments and helical structures; to divide; to ‘mate’;..to transform chemical energy into mechanical motion.

…”In 1959, Daniel Eley, at Nottingham University, proved that dried proteins, amino acids, and porphyrins are…semiconductors. In 1962, Roderock Clayton…at Oak Ridge [Nat’l Lab] found that photosynthetic tissues in living plants behave like semiconducters. In 1970 Alan Adler, at the New England Institute, showed that thin films of porphyrin do also. (p143-144).

…”Adding thin films of porphyrins to commercially available photovoltaic cells increases the voltage, current, and total power output. (p145). The properties that make porphyrins suitable in electronics are the same properties that make us alive… The secret lies in the highly pigments, fluorescent molecule called porphyrin. Strong pigments are always efficient energy absorbers, and if they are also fluorescent, they are also good energy transmitters… Porphyrins are more efficient energy transmitters than any other of life’s components.

“There is one more place these surprising molecules are found: in the nervous system, the organ where electrons flow. In fact, in mammals, the central nervous system is the only organ that shines with the red fluorescent glow of porphyrins…under ultraviolet light. These porphyrins…occur, however, …not in the neurons themselves, the cells that carry messages from our five senses to our brains, but in the myelin sheaths that envelop them—the sheaths whose…breakdown causes one of most common and least understood neurological diseases of our time: multiple sclerosis. It was orthopedic surgeon Robert O. Becker [‘The Body Electric’] who, in the 1970s, discovered that myelin sheaths are really the electrical transmission lines.

“In a state of health the myelin sheaths contain primarily two types of porphyrins…complexed with zinc. The exact composition is crucial. When environmental chemicals poison the porphyrin pathway, excess porphyrins bound to heavy metals, buildup in the nervous system as in the rest of the body [where illness like polio (acute flaccid paralysis) or skin lesions occur]. This disrupts the myelin sheaths and changes their conductivity which, in turn, alters the excitability of the nerves they surround. The entire nervous system becomes hyperreactive to stimuli of all kinds, including electromagnetic fields.

“In the nineteenth century, anatomists…supposed that [myelin] must have only a…’supportive’ role, protecting the ‘real’ nerves… They named them glial cells after the Greek word for ‘glue’ [as in gliadin and gluten so-named]… From then on, glial cells were thought to be little more than packing material. Most biologists ignored the [finding] by German physicist Rudolph Virchow in 1854, that myelin is a liquid crystal.

…”However…Becker found quite another function for the myelin-containing cells and took another step toward restoring electricity to its proper role in the function of living things. (p146-147) Becker began to pursue the ideas of Albert Szent-Gyorgyi, thinking that if proteins were semiconductors, maybe bones were too, and maybe electron flow was the secret to the healing of fractures… (p148)

“Once having established that the signals that trigger regeneration are electrical and not chemical in nature, these scientists were in for another surprise. For the DC potentials of the body…[that] are necessary…for regeneration…growth, healing, pain perception and even consciousness, seemed to be generated not in the ‘real’ nerves but in the myelin-containing cells that surround them—the cells that also contain porphyrins. (p151)  …The cells that biologists had considered merely insulation turned out to be the real wires. (p152).

“Everyone knows that the brain consumes more oxygen than any other organ…  [An] Italian team confirmed in 2009…that as much as ninety percent of that oxygen is consumed…by the myelin sheaths… (p153)

“In France, liver cancer has been found to be 36 times as frequent in people carrying a gene for porphyria as in the general population. In Sweden and Denmark the rate was 39 times as high, and the lung cancer rate triple the general rate. Chest pain, heart failure, high blood pressure, and EKGs suggestive of oxygen starvation are well-known in porphyria. (p156) …Insulin levels are usually abnormal… The protean manifestations of this disease, capable of affecting almost any organ, are widely blamed on…a deficiency of heme. Indeed, no porphyrin expert has offered a better explanation.” (p157), The Invisible Rainbow, by Arthur Firstenberg, 2017.

*

“Under natural conditions, as they existed before 1889, intense VLF activity, leading to electron rain and the shifting of the Schumann resonances, occurred only during geomagnetic storms. Today, the magnetic storm never ends.” –A.F.

 

April 11, 2020

Genetically Modified: Tag, You’re IT

*

*

“The history of biotechnology is not well known. It comes as a surprise to most people to find out that it was not a scientific discovery. Genetic engineering technology was deliberately devised by a small group of people who were looking for a way to manipulate life according to their own designs and for their own purposes. Once they identified genes as a material basis for life, they figured out how to manipulate them, using recombinant DNA technology, and they immediately found commercial uses for their products.

   “The underlying conceit is what MIT science historian Lily Kay calls the “molecular vision of life.” It came about, she says, as the result of the effort of a few scientists, along with their academic and philanthropic sponsors, who had a shared vision about how they could use genes to reshape science and society. Kay says this small group of people laid claim to DNA as a means to an end, once they decided that it was ‘the secret of life.’

   “This new ‘protein paradigm’ reduced the enormous complexity of life down to a few component parts and provided a biological basis for the idea that microorganisms could be engineered. This was the ‘new biology.’  …And it offered those who adopted it a previously unimagined mastery over both nature and society.” –pp8-9, Uncertain Peril, by Clare Hope Cummings, 2008

*

“The desire for patents and profits sweetened the prospects for all concerned. Kay says the long-standing boundaries between what was ‘public’ and what was ‘private’ were erased as corporate and government interests merged… [And] what emerged was a matrix of control. Biotechnology began to dominate scientific research… In the end, it’s been the triumph of what Kay calls the ‘technocratic approach to life.’ …Whether it was intended or not, the way biotechnology was developed has resulted in a relatively small number of players achieving an unprecedented and overarching level of control over life on earth. It brings to mind the words of Justice William O. Douglas, who warned that ‘ as nightfall does not come at once, neither does oppression. In both instances, there’s a twilight where everything remains seemingly unchanged, and it is in such twilight that we must be aware of change in the air, however slight, lest we become unwitting victims of the darkness.’ “ –pp10-11, ibid.

*****************************

“Can a microscopic tag be implanted in a person’s body to track his every movement? There’s actual discussion about that. You will rule on that –mark my words—before your tenure is over.”U.S. Sen. Joseph Biden, asked during Senate Judiciary Committee hearings on the nomination of John Roberts to be chief justice of the Supreme Court.

*

“We can take proteins, real biological machines, and make them part of a working microelectronic circuit.” –Aleksandr Noy, scientist at Univ. of California Merced, co-author of ACS Nano Letters

*

The source of the two quotes above is from the book “Forbidden Gates” by Tom and Nita Horn, published in 2010. The subject and subheading is “How genetics, robotics, artificial intelligence, synthetic biology, nanotechnology, and human enhancement herald the Dawn of Techno-Dimensional Spiritual Warfare and includes this scenario from Nita: “She asked if the biblical mark of the Beast might be a conspiracy employing specific implantable technology only now available. Her theory was gripping. An occult elite operating behind the U.S. government devises a virus that is a crossover between human and animal disease –let’s say, an entirely new and highly contagious influenza mutation [supposing the 2010 publication was directly referencing the H5N1/H1N1 ‘event’ over years 2005-2009] –and intentionally releases it into the public. A pandemic ensues, and the period between when a person contracts the virus and death is something like like ten days. With tens of thousands dead in a few weeks and the rate of death increasing hourly around the globe, a universal cry for a cure goes out. Seemingly miraculously, the government then steps forward with a vaccine. The only catch…given the nature of the animal-human flu, the ‘cure’…rewrites one’s genetics so that the person is no longer entirely human…[and by] receiv[ing] this antidote would become part ‘beast’…thus…’Mark of the Beast’

At the time of reading Forbidden Gates, shortly after publication, it was with full awareness that a bioinformatics database called BEAST already existed. It’s in the flu notes, posted on this blog. Biblical ‘scripting’ is, to me, the ominous and perversely-expressed harbinger of the spiritual warfare reality.

As far as I’m concerned, a mass of human tagging began with the 1950s polio vaccines and the introduction of the monkey virus ‘contaminant’ SV40, simian virus #40, in the Salk and Sabin formulas. It’s not a gift of altruism that ongoing worldwide polio vaccination has been the goal of Bill Gates and the foundation, though current vaccines are SV40 free. The theory is basically this: Post-Hiroshima biological surveillance and data collection was mandated to the medical team of the Atomic Bomb Casualty Commission (ABCC) on the suggestion of 100 years duration. A multigenerational biological tag was the only means then available, lacking nano-device alternatives, and its expected implementation was imminent after 1950 when the ABCC organized for its longterm assignments. Nuclear testing, which began in Nevada in 1951, expanded the mandate to the U.S. population and beyond. Polio-vaxxed Boomers and their descendents are a potentially trackable demographic. Some military-use nonpolio vaccines of the era also contained SV40. Adding to this view is code for the infective SV40 particle, the T-antigen short form –T-ag.  Tag, really. Is that nerd humor?, an ominous and perversely-expressed harbinger?— or just in-your-face who-doo for future surveillance in ABCC’s project management? The ABCC, of course, is long defunct, but SV40 rages on, fascinating as a subject in the development of  biotech. For now, as a model of tagging derived entirely by my own thoughts on it, I’m going with ‘in your face’ and on the hook. The journey of SV40 through research labs as a ‘recombinant’ gene factor parallels the growth of the biotech industry.

*********************************

Who owns your DNA? According to Claire Cummings 2008 book, Uncertain Peril, “Twenty percent of the DNA in the human body is now patented. Many of the genes in your body are already owned by someone else, most likely a pharmaceutical company or an institution. The Regents of the University of California, for instance, own eighty-nine human genes.” –p75, ibid.  Little doubt attends my speculation that an increase in holdings over the last decade put aggregate institutional ownership of human DNA at or near a  majority of stock—live stock. Somehow, between the first successful 1980 “patent on life” case for a genetically-engineered bacterium and the present, ownership of your essential constituents have been transferred to private parties. Quantitatively speaking, only about 10% of ‘you’ is human anyway, long provoking the question of “what is human?” and, by extension, “what is ‘yours’ and ‘not yours’?,” and “when does ‘yours’ become ‘not yours’?”

So far, every time I check into it, the human DNA legal trend is “not yours” and when applied to medical treatment definitely “not yours.” The immune system wrestles with this every day, tasking “self” and “not self,” so I’ll try to work out a parallel here.

*

In the meantime, an example of “not yours”:  “…when the Supreme Court ruled on the [patent] case of Ananda Mohan Chakrabarty, a scientist working at General Electric who’d created a bacterium genetically engineered to consume oil and help clean up oil spills…[his] lawyers argued that since normal bacteria don’t consume oil, Chakrabarty’s bacteria weren’t naturally occurring –they only existed because he’d altered them using ‘human ingenuity.’ Chakrabarty’s victory opened up the possibility of patenting other living things, including genetically modified animals and cell lines, which didn’t occur naturally outside the body. And patenting cell lines didn’t require informing or getting permission from the ‘cell donors.’ “   The bold-type emphasis is my addition to the words of Rebecca Skloot from the book ‘The Immortal Life of Henrietta Lacks’ whose cervical cancer tumor specimens became the famous ‘immortal’ HeLa cell line in 1951.  Skloot mentions another cell line called ‘Mo,’ harvested by a UCLA doctor in 1976 from the abnormal spleen of a patient named John Moore who suffered from hairy-cell leukemia. “A normal spleen weighs less than a pound; Moore’s weighed twenty-two.” –p199  Some years later, during his ongoing medical follow-up which involved long distance travel to UCLA, Moore’s suspicion was aroused when he attempted to change the arrangements of his medical visits to be closer to home. Accompanying offers of plane tickets and a stay at the Beverly Wiltshire hotel, was  “a  new consent form that said: ‘ I (do, do not) voluntarily grant to the University of California all rights I, or my heirs, may have in any cell line or any other potential product… developed from the blood and/or bone marrow obtained from me.’ “   He circled ‘do’ but at his next visit when the consent form was presented again he circled ‘do not’ and soon learned that ‘Mo’ was reaping a windfall. In 1984 Moore filed suit against the physician and UCLA. His doctor, patent in hand, stood to personally gain $3.5 million from the cell line which “at that point [had] its market value estimated to be $3 billion…  Scientists are quick to point out that John Moore’s cells were exceptional, and few cell lines are actually worth patenting. Moore’s cells produced rare proteins that pharmaceutical companies could use to treat infections and cancer. They also carried a rare virus called HTLV…which researchers hoped to use to create a vaccine that could stop the AIDS epidemic. Because of this, drug companies were willing to pay enormous sums to work with his cells.” –pp201-202, ibid.

Moore lost on the first round, then appealed in 1988 and won. His doctor subsequently appealed and won the ‘right to use patient tissues’ and finally “Nearly seven years after Moore originally filed suit, the Supreme Court of California ruled against him in what became the definitive statement on this issue: When tissues are removed from your body, with or without your consent, any claim you might have had to owning them vanishes… [The court] said that ruling in Moore’s favor might ‘destroy the economic incentive to conduct important medical research,’ and that giving patients property rights in their tissues might ‘hinder research by restricting access to the necessary raw materials’…” –p205, The Immortal Life of Henrietta Lacks, by Rebecca Skloot, 2010

*****************************

The roots of this blog are mentioned in a post called “A Few Words About the Agenda” from November 2009:

“….I started this blog in July09 in the midst of a “pandemic” that is not happening because of a deep foreboding that the Controls being sought are nearly in hand. There is no rational/logical explanation for things like mandatory vaccines, forced healthcare, carbon-footprint permits and the like when it flies in the face of experiential science. What else can it be but the consolidation of the Plan? Every article here in my blog is telling an aspect of this story; One story about One World, where everything coalesces under…the oligarchy…[until] they own you… In ‘their’ future.. ‘we’ are to be made less than fully human to eliminate their competition…  today achievable in physical fact.  How will we be ‘less than human’ –perhaps by becoming programmable DNA computers. Take a look: http://en.wikipedia.org/wiki/DNA_computing

…. The impetus in DNA computing, according to Ehud Shapiro of the Weizmann Institute speaking in 2003, is to find a “molecule that can recognize, cut and join DNA sequences in specific ways”, what he suggests will be “designer enzymes…that can do things and go to places that silicon can’t –such as inside our cells to make and control drugs.” http://www.usc.edu/dept/molecular-science/index.html . Phage and virus already do this naturally –enter our cells and install a program– so the question is begging about the ability of virus-like ‘nano-machines’ harnessed for the activity of synthesizing specific enzymes to run an assembly program. If you were going to ‘assemble’ a DNA computer capable of replicating and replacing its own parts, wouldn’t the ideal machine have an immortal and unlimited source of those parts? Cancer cells are just such an immortal cellular anomaly. A pharmaceutical-generating program that can control cancer cells in the self-performance of chemotherapy has the capacity I would think of becoming an immortal DNA computer, capable of [reproducing] itself with endless ‘perfect copies’ while keeping the overproduction of those cells in check…  Cancer cells may become the needed raw material for constructing immortal bio-bot computers. The staggering potential of DNA computing forecast by Leonard Adleman is that “One gram of DNA can store as much information as a trillion compact discs”. “What’s more”, states the text of the USC webpages above, “myriad DNA molecules can examine every possible [pathway] at once, rather than one at a time as in a conventional computer”. With this much incredible promise, is it likely that the DNA computing science would take a backseat? If I’m on the right track with this projection, a lot of agendas appear to be satisfied. I’m over my head here…  Explanations for the presence of sophisticated and nano-sized materials in food, vaccines and chemtrails are not forthcoming and yet they are turning up in products of every description. At the atomic level, organic, inorganic and cellular materials have new and different properties, most informative of which comes from electrochemical experiments.  As in the past, this new technical platform will be maximally spun-off and exploited in some ultimate pursuit of global mastery… Our bodies will become factories for their bio-bots.

2004– “Recently, simple molecular-scale autonomous programmable computers were demonstrated… allowing both input and output… Such computers, using biological molecules as input data and biologically active molecules as output, could produce a system for ‘logical’ control of biological processes… As proof of principle, we programmed the computer to identify and analyze mRNA of disease-related genes associated with models of…cancer, and to produce a single-stranded DNA molecule modelled after an anticancer drug.” http://www.nature.com/nature/journal/v429/n6990/abs/nature02551.html

(end of self-quote from A Few Words About the Agenda)

************************************************************************************

”The goal of the government should be one level of health for all the people” —Florence Mahoney, 1965, reference on p209 of Noble Conspirator, Florence S. Mahoney and the Rise of the National Institutes of Health, by Judith Robinson, 2001

This quote is but one of the select bolshevist utterances of the incomparable ‘health’ lobbyist Mrs. Mahoney. Her principal endeavors focused on population control, mental health treatment and social engineering. The span of her nationally influential activity ranged from the 1940s to the ‘90s and “by the year 2000, the National Institutes of Health had become indisputably the world’s leading research entity across the spectrum of biomedicine.” –271, ibid.

The 2009 H1N1 pandemic-that-wasn’t brought in the government initiative called One Medicine, a cooperative paramilitary organization enlisting MDs and DVMs to jointly respond to public health issues. In essence, the One Medicine goal is to devise common protocols of treatment for humans and animals –symbolically, if not actually, leveling the livestock. In biotechnology this is possible because of  genetic ‘homology’, the cross-species similitude of gene chemistry. In natural settings, homology in viruses, viral vectors and host receptors leads to transmission –and in the case of infectious transmission from animals to humans, known as ‘zoonosis’ (zoh-ahn’-nah-sis). I’ll have to check if ‘humanosis’ or ‘homonosis’ is in the lexicon yet. We’re certainly making the animals we love and depend on very sick with ‘public health’ protocols.

Any government that determines the nature of my human physical matter, whether inside or outside the body, to be “raw material” for “important” economic exploit represents neither myself nor the authority to which I submit.

*

Compare…..

Industrial GMOs have a running track record in agriculture, the subject of Uncertain Peril, sufficiently parallel in models of business and ethics to apply to human engineering. I put in the bracketed [noun] substitutions to make a point:

“There are five solid reasons that genetic engineering is not right for agriculture [medicine]. One: it’s bad science. It was developed on the basis of flawed assumptions which have since been discredited by the scientific community. Two: it’s bad biology. It was deployed without regard for its potential for genetic contamination and risks to human health. Three: it’s bad social policy. It puts control over seeds [medical treatment] and the fundamentals of our food and farms [healthcare] into the hands of a few corporations who have their own, not our, best interests in mind. Four: it’s bad economics. After billions of dollars and thirty [now forty and more] years, only a few products have been commercialized, and they offer nothing new. No one asked for genetically modified organisms (GMOs), and given a choice, consumers would reject them. Five: it’s bad farming [healthcare]. GMOs don’t address the real issues plaguing agriculture [humans]; they’re designed to substitute for or increase the use of proprietary weed and pest [drug] control chemicals. Patented and genetically altered seeds [pharmaceuticals] perpetuate the very worst problems of the industrial food [health] system, and they are undermining the autonomy of the farmers [doctors] who use them.”

*********************8******************************

Underlying Causes and Coronavirus

*

‘You Are What You Eat’ was a government information campaign of the 1970s when biotech was gaining ground in agriculture. The learning program put an emphasis on the benefits of whole grain –certain of which are the gluten/gliadin containing wheat, rye, and barley which we now know compromise the blood-brain barrier, as the glyphosate (Round-up, Agent Orange) herbicide then being introduced compromises the gut by inducing lesions. (That’s subject for another post). The point here is that my earlier influenza research demonstrated that the microbiologists of the last century discovered toxic gut bacteria in the lungs of flu patients, determining it to be the cause of respiratory infection.

Plus this: “…In this account of the discovery of influenza [virus, c1933], researchers at ”Mill Hill” near London accidently infected their lab ferrets with their own flu. [humanosis?] At the time they were experimenting with ‘distemper’, developing a vaccine for the flu-like illness in dogs.”   –see the margin list for “Influenza Special”. If there’s a relationship of gut bacteria to respiratory infection in dogs, I didn’t look for it then, but the common viral agent of contagious gastroenteritis in dogs is coronavirus.  From the Merck Veterinary Manual, 1991 edition: “Coronaviral Gastroenteritis, A highly contagious GI disease of dogs of any age… Canine Coronavirus (CCV) is an enveloped, single-stranded RNA virus antigenically related to the feline coronaviruses (feline infectious peritonitis virus and feline enteric coronavirus) and to transmissible gastroenteritis virus of pigs… Lesions in experimental infections usually are not severe…[but] Naturally occurring cases, especially those with mixed infections, can have severe lesions with frank hemorrhage in the intestinal mucosa…” The 1991 Merck Vet. lists only four species with economically significant coronavirus problems: dogs, cats, pigs and turkeys, but if there are clues in microbe-hunting about CoV transmission, mixed-infections, and treatment, the recommended treatment for Kennel Cough in dogs is vaccines for distemper and parainfluenza (or paramyxovirus infection, PMV) –respiratory infection in turkeys, “which suggests the respiratory tract may be the initial site of infection.” –p1564, ibid.  Bird flu? Do turkeys cough? “The transmission between turkeys is unclear,” so I’ll let that go for the time being. However, turkeys take a wallop with coronavirus! “Coronaviral Enteritis of Turkeys: An acute, highly contagious disease of turkeys characterized by sudden onset… Mortality may be high… The causative agent is a coronavirus, but the clinical disease is complicated by other intestinal viral and bacterial infections… Cold weather, especially freezing, increases survival of the virus.” –p1554.  We’re being told that too, yes? Must be One Medicine on the job. No vaccine for turkeys, says Merck…just quarantine, social distancing, disinfection…  A little global warming would be handy.

A  very different outcome of coronavirus in pigs is the ‘causal agent’ of a frightful disease called ‘vomiting and wasting disease’ (VWD), otherwise known as Coronaviral  hemagglutinating encephalomyelitis virus (HEV) –a brain infection—where “Neonatal pigs become dehydrated, cyanotic, comatose and die…[If] the virus enters a susceptible herd with neonatal piglets, morbidity and mortality may be high… Replication first takes place in the nasal mucosa, tonsils, lungs, and to a very limited extent, in the small intestine [after spread by aerosol]. From these sites of entry, the virus invades…the peripheral nervous system and subsequently spreads to the entire brain stem…” –pp388-389, ibid. I’m posting this not to scare you but to show a greater range of coronavirus. In North America, western Europe, and Australia, where HEV occurs, “Pigs are the only natural host.” This is the kind of information that provokes my attention for bioweapon potential –but ‘bioweapon’ within the meme of low-intensity conflict scenarios. Our ‘common cold’ coronavirus is everywhere.

 

Turn back the clock for a minute on the official U.S. response to SARS in May 2003:

…”Thank you, Mr. Chairman and members of the Committee. I am Jerome M. Hauer*, Acting Assistant Secretary for Public Health Emergency Preparedness. I appreciate this opportunity to share our Department’s response to the SARS virus within the context of public health emergency preparedness… we have reason to be encouraged by the response to SARS for several reasons. First, the identification of the agent that causes the disease was completed in record time. CDC identified the coronavirus within a few short weeks of receiving the first specimens… We are partnering with industry to organize a full-court press on vaccine development… The Command Center maps the distribution of SARS cases across the globe with geographic information system software for use during our planning discussions. The Command Center did not exist a year ago – it became operational last November [2002]… I recently co-chaired a meeting of the Council of Governments with Mike Byrne of the Department of Homeland Security to bring together health professionals from across the national capital region to aggressively prepare for an outbreak of the SARS virus here… the Department is implementing an aggressive research and development program to develop and acquire biological, chemical, nuclear and radiological countermeasures… The most exciting news in the R&D arena is, of course, Project BioShield, announced by the President on February 3, 2003.” http://republicans.energycommerce.house.gov/108/Hearings/05072003hearing917/Hauer1433.htm

*Jerome Hauer, you may remember, was on hand for commentary to major NYC TV media the morning of 9-11, providing the tip that ‘Osama bin Laden’ did it. Hauer was then an officer of New York’s emergency operations center and pKroll security, but his earlier career was in bioweaponry. He directed the neighborhood pesticide-spraying campaign against West Nile Virus when it emerged in NYC in 1999.

*

To our sorrow, the legal decoupling of “healthcare” from the biotech industry never happened. It could have, should have, happened long ago in the 1950s and ‘60s amidst the Cold War when advancing biotechnology was pressing countermeasures (like Hauer’s B-C-R protocols) into military operations. The subsequent infiltration and takeover of biotech in medicine forces the euphemism of “healthcare” with the still added integration of I.T. –Information Technology. As I see it, all a very advanced and comprehensive outcome of radiation biology, the mother of modern genetics.

*

In June of 2013, Justice Clarence Thomas (former Monsanto lawyer) wrote the decision on human DNA for the US Supreme Court that, “We hold that a naturally occurring DNA segment is a product of nature and not patent eligible merely because it has been isolated,” following more than forty years of neglect while the industry flourished. At stake was the monopolization of a natural gene by one patent holder, able to control its use in research and medicine. The decision opened the pool and, interpreted by the industry, “also said that synthetic genetic material could be patented” –synthetic human DNA. Four years before this decision, August 2009, I posted “Fabricating DNA Evidence” quoting “Scientists in Israel have demonstrated that it is possible to fabricate DNA evidence…if they have access to a DNA profile in a database… without obtaining any tissue from [a] person. ‘You can just engineer a crime scene,’ said [Dr.] Dan Frumkin… ’Any biology undergraduate could perform this.”

*

“Synthetic biology is unregulated and self-governing. No one knows what could occur when synthetic organisms are intentionally and unintentionally let loose into the environment. Creating genes that don’t exist in nature is a dangerous business and there is no way to predict how they will behave in living systems.” –p266, Foodopoly, 2012, by Wenonah Hauter (Food & Water Watch)

*

So, while I’m ‘CoVing-in’ with extra spare time, I’m dwelling on the tagging, tracking, and surveillance milieu of our present situation juxtaposed with notions of human rights because biotechnology is rewriting the genes of our Constitution and incapacitating the judgements therefrom:

 

FYI, The Human Rights which we extend around the globe, and teach to students of ‘public health’, “include but transcend the conventional classes of human rights…in a remarkable variety of international human rights and humanitarian laws… Never before…has there been such universal recognition of the claim that everyone…is entitled to rights—in particular, what have been aptly called ‘life integrity rights.’ These include the right to life, the right to personal inviolability—not to be hurt; the right to be free of arbitrary seizure, detention, and punishment; the freedom to own one’s body and labor; the right to free movement without discrimination; and the right to create and cohabit with family.” –p39, War and Public Health, 2000 updated ed., editors Levy and Sidel.

“We hold these truths to be self-evident, that all [mankind] are created equal; that they are endowed by their Creator with certain inalienable rights… [and] that whenever any form of government becomes destructive of these ends, it is the right of the people to alter or abolish it… Prudence, indeed, will dictate that governments long established should not be changed for light and transient causes; and accordingly, all experience hath shown, that mankind are more disposed to suffer, while evils are sufferable, than to right themselves by abolishing the forms of government to which they are accustomed. But when a long train of abuses and usurpations, pursuing invariably the same object, evinces a design to reduce them under absolute despotism, it is their right, it is their duty, to throw off such government and to provide new guards for their future security. The history of the present… is a history of repeated injuries and usurpations, all having, in direct object, the establishment of an absolute tyranny… To prove this, let facts be submitted to a candid world…” A Declaration by the Representatives of the United States of America… July 4, 1776

*

********************

…part two coming soon…

********************

 

 

March 28, 2020

Germ Games

“Germ Games”, said Bill Gates in 2009, should replace “War Games”.  In 2010, Gates put his money where his mouth is:

*January 29, 2010 [news] “Gates Foundation Pledges $10 Billion to Vaccine Research” http://www.washingtonpost.com/wp-dyn/content/article/2010/01/29/AR2010012903953.html  “DAVOS, SWITZERLAND — The Bill and Melinda Gates Foundation will donate $10 billion over the next decade to research vaccines and make them available to the world’s poorest countries, the Microsoft co-founder and his wife said Friday…’We must make this the decade of vaccines,’ Bill Gates said in a statement.”

February 2010 — Bill Gates: “If we do a really great job; on new vaccines, healthcare, reproductive health services –we could lower [population] by 10 or 15 percent…  http://www.youtube.com/watch?v=6WQtRI7A064&feature=player_embedded

* posted at www.polioforever.wordpress.com/sabin-vaccine-institute

COVID-19

March 16, 2020 –The AP* print news out of Seattle, published in my town paper the next day on March 17, is that Microsoft’s home city is delivering the first COVID vaccine (called mRNA-1273) to be tested on humans in the U.S.  Four volunteers were injected, according to the article, by Kaiser Permanente’s Washington Research Institute—two of whom were described as (#1) “an operations manager at a small tech company” and (#2)”a Microsoft network engineer”.  The other two were not described. The experimental vaccine is targeting the CoV ‘spike’ protein: “The idea: The body will become a mini-factory, producing some harmless spike protein.”  They hope. The new “vaccine candidate, code-named mRNA-1273, was developed by the NIH and Massachusetts-based biotechnology company Moderna Inc…”  And, P.S., the article says, “There’s no chance participants could get infected because the shots do not contain the coronavirus itself.”  The vaccine is attempting to get cells to manufacture only the “harmless spike protein” on the grounds it will prep the immune response. Dunno, folks.  The sophistication of new mRNA vaccines, in experimental use to control cancer, suggest to me that the ‘platform’ is tailored to nanodevices –I’m in for  lessons.

*AP article by Lauran Neergaard and Carla K. Johnson

Moderna Inc. in Cambridge, MA,  has “strategic alliances…[ with various biotech/pharmaceutical companies], Defense Advanced Research Projects Agency [DARPA] and the Bill and Melinda Gates Foundation.https://finance.yahoo.com/quote/MRNA/profile    …for sure!

The notable CoV Boston outbreak which occurred after a hotel seminar and produced about 8 cases, occurred among employees of another Cambridge biotech firm,  Biogen.  Biogen was a Cambridge biotech start-up pioneer,  founded by  Nobel Prize-winning biochemist Philip A. Sharp, whom I discovered as an SV40 researcher, the monkey virus contaminant in the Salk and Sabin vaccines of the 1950s-60s, in the field of recombinant DNA. Biogen was established in 1978 when the recombinant DNA controversy reached a peak in the public eye. Sharp got his Prize later in 1993 for discovering  methods of RNA-splicing.

RNA splicing

RNA,  ribonucleic acid, is the base of our complement  gene system , coded to carry the template in our cells for the production of more RNA, DNA and proteins. In a string of RNA codons, some noncoding sections may exist which can be subject to removal in a process of “cut and paste” editing, either by natural means or by genetic engineering,  producing new variants of messenger RNA (mRNA), the blueprint instructions.

Spliced mRNA from viruses have a prodigious range of functions as tools in molecular construction, modified to improve their utility :  .… “our new method is expected to rapidly expand our knowledge on RNA splicing mechanisms for a wide range of viruses.”   https://www.nature.com/articles/s41598-018-21190-7 This particular article from nature.com, by chance, has an all-Chinese team of scientists.

 

Corona virus has (in other citations of many years ago) popped up as a useful ‘cleaving’ agent for bioengineers.  If believing someday we will all be ‘mini-factories’ in the post-industrial nano-bio paradigm, seeding us with their raw materials is inevitable like rain falling from the sky.  They’re testing us for uptake and the language is frank.

 

…And then… we merge with the Internet of Things. We’re doing it now.

*********

I recommend the brief page on the Sabin Vaccine Institute, posted in 2010, linked above at www.polioforever.wordpress.com  and supported by the Gates Foundation. On the page:

“Founding Chairman of the Sabin Vaccine Institute H. R. Shepherd – expert on aerosol medication; author of “Aerosols : Science and Technology”; founder of Aerosol Techniques Inc (renamed Armstrong Pharmaceuticals, acquired by Medeva PLC)–  had this to say on the receipt of a $100 million gift from the Gates Foundation :

“Vaccines are the most powerful tool available to equalize the health of human beings in every corner of the world” he said. “Enlightened leaders understand the power of vaccines to help bring peace and opportunity to the most troubled places in our world. Vaccines are the microchip that will revolutionize healthcare”  http://www.sabin.org/files/SVR_No1_Vol2.pdf 

*The Gates, we know, is heavily funding geoengineering. It also holds stakes in the Svalbard seedbank.

 

The Bottom Line is that biology models ‘template’ all of our systems. Too many nonadaptive and artificial inputs in any system will crash it eventually, even if the ‘small stuff’ appears tolerated –what’s the saying?—it’s all small stuff. Watch out for that small stuff. It’s the foundation upon which you live.

 

****************************************************************************

Germ Games vocabulary:

 

Social distancing : def., (1) standing six feet apart. That’s our “burial” distance; (2) also less than average height so if you fall over you won’t ‘domino’ the queue at the grocery outlet; (3) if you’re sitting, three feet is okay.

 

Stay-at-home orders: def.,(1) self-quarantine, alone together, work online, blah, blah; (2) kinder, gentler martial law that induces lifestyle changes; (3) in 2020 stay-at-home orders still applies to calling or texting ahead for Take-Out. Get there, pick it up, stay in your car and/or leave the area.

 

Take measures: def., (1) wear masks, gloves, and protective garments in public; (2) establish online contacts with essential services. (3) practice the aforementioned above. (4) If you’re a business, establish online contacts.

 

Learn your lessons: def., (1) compare today with what you learned from recent Ebola, H5N1 and H1N1 flu epidemics, especially if you didn’t get sick –go back and review. Talk about HIV or prior SARS is obsolete, stick with Ebola and Flu. (2) watch zombie apocalypse movies at home; (3) if you’re a student, sign up for online school.

 

Spike: def., (1) corona virus has a spike. Use the word as often as possible to help people ‘get it’.

 

Grief: def., (1) pandemics are confusing and frustrating; (a) people need help with orderly thinking. Be nice but give orders and don’t say things like “Bend over and kiss your cash goodbye.” (b) Offer alcohol-based wet-wipes if you have any and encourage counseling online.

 

 

 

 

December 4, 2017

Petkau Effect: devastating low dose radiation

In 1972, more than 46 years ago, the nuclear science world got its first definitive proof of the devastating effects of low-level radiation on humans and the environment. The messenger of that news was Dr. Abram Petkau who worked for the Canadian nuclear establishment. His work lives on as the Petkau Effect.

Dr. Abram Petkau*

**
I recently obtained a book The Petkau Effect; Nuclear Radiation, People and Trees by Ralph Graeub, updated fourth edition 1992. Dr. Ernest Sternglass provided a lengthy introduction including a description of damage to biological cells:

“…Petkau and his co-workers showed that the cell membrane damage was due to a completely different biological mechanism than the direct hit on the DNA molecules in the nucleus of cells that had been observed at the high doses and dose-rates of atomic bomb detonation or medical exposures. It turned out that the cell membranes were destroyed as the result of the action of a negatively charged, short-lived form of ordinary oxygen, the so-called O2-negative free radical, produced by the absorbed radiation from the life-giving oxygen dissolved in the surrounding fluid. This highly toxic form of oxygen diffused to the outside of the membranes, where it initiated a chain reaction that dissolved the membrane in a matter of minutes to hours, causing the cell to leak and die.
“It became clear that a single O2negative molecule was sufficient to destroy an entire cell, so that only a handful needed to be produced per cell-volume at very low dose rates. But at high dose rates, many millions would be formed in the same volume in the lifetime of the molecule. This was a form of overkill, much like the case of a balloon where a single dart is enough to destroy it, and throwing millions of darts at it only represents a waste of energy. In fact, the more free radicals are created in a given volume, the more they tend to run into each other causing them to become deactivated to harmless ordinary oxygen. Thus, per unit of energy deposited in living tissue consisting of cells, high doses given at the rate of 10,000 rads per minute were found to be 100 billion times less efficient in destroying a cell than at one ten-millionths of a rad per minute, the rate at which we experience background radiation.
“As described in The Petkau Effect, the consequence of the enormously greater efficiency of radiation at low, as compared to high, dose rates is that the dose-response curve rises very rapidly at small doses…
“…As discussed by Graeub, the indirect free radical type of damage that dominates at low dose-rates is particularly serious for the cells of the immune system, which must be constantly renewed from their progenitors in the bone marrow. This is especially true for strontium-90 and other bone-seeking isotopes chemically similar to calcium that concentrate in bone and emit relatively long-range beta particles or electrons…”

*

*Dr. Ernest Sternglass:

*** “Graeub presents enormously important but little-known evidence that nuclear plant releases are also contributing to the production of acid rain, ground-level ozone and the death of forests… the death of trees has reached epidemic proportions…   Again, the indirect effects of radiation through the production of free radicals on ordinary air pollutants and the cell membranes of plants seem to be the reason…” [into p.25, The Petkau Effect]

*The Petkau Effect:*

“In 1972 the scientist Abram Petkau at the Canadian Atomic Energy Commission’s Whiteshell Nuclear Research establishment in Manitoba, made an accidental discovery deserving of the Nobel Prize. Petkau irradiated artificial cell membranes under water, using phospholipid membranes which are similar to the cell membranes in living cells. He discovered that if the irradiation continued over an extended period, the membranes would tear after a much lower total absorption of radiation dose than if this total dose were emitted in the form of a short burst, as used for x-ray film. A living cell consists of a cell membrane and a cell nucleus. But the cell membrane is not only there to hold the watery cell plasma together; it has many important functions in biological processes. These tasks have been compared with those of an entire industrial corporation. Thus, intact cell membranes are essential for a healthy life. What Petkau discovered was the following: A short term irradiation of 26 rads per minute (i.e., a high dose rate) from a large x-ray machine required the high total dose of 3,500 rads in order to destroy the cell membrane. However, with protracted radiation of only 0.001 rads per minute (i.e., a low dose rate) from radioactive table salt (Na22Cl) dissolved in water, a total dose of only 0.7 rads was required to break it. Thus, in the case of low-level, protracted irradiation, a 5,000-times smaller total dose was necessary. This was truly an incredible discovery.” [p86]

*

“Ionizing radiation affects the structure and chemistry of the cells. Ions [or electrically charged molecules] are formed, and..may be split apart, forming radicals. Radicals are pieces of molecules that are chemically very aggressive and can form new compounds which are foreign and in some cases toxic.” [p26]
“In the cell fluid, which contains dissolved oxygen, the radiation can cause the formation of a highly toxic, unstable form of oxygen. These so-called ‘free radicals of O2- [or superoxide anions] are attracted by the cell membrane, where they set off a chain reaction that successively oxidizes the molecules of the cell membrane; this weakens or even destroys the membrane. Thus, unlike the case of the cell nucleus [containing DNA], the damage is not the direct result of radiation; rather it occurs indirectly, as the result of the ‘free radicals’ created by the radiation.
…The fewer free radicals present in the cell plasma, the greater their efficiency in producing damage. This is because the free radicals can deactivate each other to form ordinary oxygen molecules. Thus, the fewer free radicals created by radiation in a given volume –and smaller doses create fewer of them– the greater their chances of reaching..the cell wall without first being subjected to a recombination.
Conversely, the more free radicals created..the faster they recombine and become ineffective before they can reach and damage the membrane. In addition, there is a further effect. Cell membranes generate an electrical field in the cell plasma which attracts negatively-charged molecules such as the highly toxic free radical. Computer calculations have shown that the greater the concentration of free radicals, the weaker the attraction by the electric field. Thus, if the concentration of radicals is high, [they] are even less capable of reaching the cell wall than if the immediate concentration of radicals is very low.” [p88]
“Numerous scientific studies of the past..have shown that indirect cell membrane damage must also be effective in biological systems, even at the most minimal doses of 10-100 mrads [millirads] (0.1–1mGray), i.e. in the range of natural radiation, fallout and emissions from a nuclear power plant.” [p90]

**

* Low-dose radiation effects on insects living near nuclear power plants are under close observation by scientific illustrator Cornelia Hesse-Honegger, who says in her own words: “When Chernobyl happened, I knew it was time for me to act… I traveled to Sweden, which was also affected by the radioactive plume, to look for mutated bugs… They stay near the same piece of ground for generations, making them excellent subjects for the study of..prolonged radiation… I found terribly deformed insects, even though the levels of radiation there were relatively low… In the southern part of Switzerland, which was highly irradiated by Chernobyl, I collected three pairs of [fruit] flies and bred them in my kitchen… From the first generation on, the flies were deformed. In 1988, I published this and similar data…  In 1992, I decided to start a systematic study of the effects of nuclear power, traveling to nuclear plants around the world and gathering..bugs living around them… What I found was..a consistently higher rate of deformations.”  http://www.nautil.us/issue/14/mutation/why-i-traveled-the-world-hunting-for-mutant-bugs/

***                                 

“The first signs of forest death coincide with the first appearance of artificial radioactivity in our environment. [p162, The Petkau Effect] …Of all civilization-induced pollutants, radionuclides have had the highest rate of increase relative to their natural rate of production. The military and civilian use of nuclear energy is responsible for the massive increase in the level of radioactive pollutants… For instance, the atmospheric concentration of krypton85 has increased by millions…  [L]arge accumulations of radionuclides and their daughter products in leaves, needles and the soil due to the bomb fallout of the ’50s and ’60s have been proven with certainty. This is also true for plutonium, americium, tritium and carbon14… [It’s] now determined that forest death has been strongly increasing throughout the Northern Hemisphere since the time that the long-lived radionuclides from the A-bomb tests started spreading to the root areas of trees… Thus, the observed delay of the damage… [pp155-157] “Reduction of its concentration in the air by dry sedimentation or wet precipitation is only minimally successful. This is a very serious factor, for 97% of the krypton remains in the atmosphere and is eliminated only by..radioactive decomposition which may take decades… The long half-life of krypton means irresistable build-up in the environment… If the krypton reaches even one percent of the maximum permissible concentration in the air (300 nCi/m3), measurable global changes in the electric conditions of the atmosphere will begin to occur… It might be possible for lightning bolts in widely separated regions to be connected by electrical feedback. This may cause unexpected changes in the weather.” [p140-141]

***                               

 

***                                                                        

“During the 1950s and 1960s, there must have been a global wave of air pollution which caused the initial damage. It was certainly not our cars with NOx emissions and photosmog that were to blame, nor is it likely that SO2 was solely responsible…[p123]
“The acid rain hypothesis [in forest death] is based on the assumption that sulphuric, nitric, hydrochloric and carbonic acids lead to chemical reactions in the soil. This liberates plant-toxic aluminum and manganese ions, damaging to root hairs…. However, if SO2 (sulphur dioxide) is dissolved in water in the lab, nearly the only thing that is produced is sulphurous acid (H2SO3) which is much weaker than sulphuric acid (H2SO4). In order to produce the very acidic sulphuric acid, it is first necessary to oxidize the sulphur dioxide (SO2) to sulpher trioxide (SO3)… It is thought that photo-oxidants such as ozone and hydrogen peroxide (generated by the effect of sunlight on NOx’s and hydrocarbons) act as catalysts or promoters of the reaction. However, artificial radioactive substances may have the same effect. Artificial radiation produces radiant energy and can directly oxidize SO2 to SO3, or form ozone from atmospheric oxygen. Radiolysis in water can form hydrogen peroxide directly, and may even produce NOx from atmospheric nitrogen. [p124]
“At a 1975 meeting of the International Atomic Energy [Agency], a number of very interesting correlations involving radioactivity were presented by the scientist K.C. Vohra of the Bhabba Atomic Research Center at Trombay, India… Vohra began with the assumption that so-called condensation nuclei are constantly forming in our atmosphere by means of chemical reactions of various substances. He was able to determine by experiment that this condensation-nucleus formation increased somewhat in the [sulphur dioxide]-rich exhaust gases under the effect of sunlight or cosmic radiation. However, when radioactive gases were released from the nuclear power plant, condensation-nucleus formation increased rapidly. The SO2 was quickly oxidized to SO3, which in turn leads to sulphuric acid, which also forms condensation nuclei much more readily.” [p125]
“Moreover, ozone can be produced in the lower atmosphere by radioactivity, utterly independent of sunlight. Thus, such radioactive inert gases as krypton85 and xenon133, which are released unchecked in all atomic fission processes, are known as effective ozone-generators.” [p132]

……….more to come: quotes from The Petkau Effect and info on oxygen free radicals…………..

June 25, 2011

Shiga-boom Shiga-boom

Shiga toxin-producing (Stx) O104:H4 killer E.coli strain:
Blame Japan
“A private biotechnology company used their DNA scanning algorithms to determine that E.coli O104 has genomic signatures specific [to]..previously found..strains of the 1996 outbreak in Sakai City, Japan… 103 pieces among the nearly one million genome fragments of the E.coli O104 are also present in phages of enterohemorrhagic E.coli isolated in 1996 in Japan… Orion Integrated Biosciences Inc. has developed this capability to scan unknown DNA fragments and point to their most likely source. The company is funded by the Departments of Defense, Homeland Security and the National Institutes of Health to apply this technology for biodefense purposes.” http://www.orionbiosciences.com/news/ORION-PR-June-05-11.pdf
…back in 1996…
“The Japanese..learned the hard way, 15 years ago, when that health-conscious society was gripped by what may be history’s worst outbreak of E.coli O157:H7. The epidemic sickened thousands, most of them children, and killed at least 12. It also showed critical weaknesses in Japan’s public health system –problems that the government spent years trying to correct. Up to that time, E.coli O157:H7 was virtually unknown in Japan… That all changed in mid-July when health officials in Sakai City, a major port city with a population near a million, received reports… Within a week, thousands were ill, hundreds hospitalized, some with hemolytic uremic syndrome (HUS), requiring kidney dialysis. And some were dying… Meanwhile, the epidemic seemed to be expanding… It was terrible timing. A few years earlier, Japan’s booming economy collapsed. Then, in 1995, an earthquake flattened the city of Kobe, not far from Sakai City. A few months later, terrorists [Aum Shinrikyo ‘yoga’ cult] released deadly sarin gas… Eventually the outbreak sickened at least 9,441 people…” http://www.foodsafetynews.com/2011/01/sprouts-and-historys-worst-outbreak/ A study paper published in 2002 reports the number of infection cases as 17,877: http://aac.asm.org/cgi/content/full/46/11/3478
*
But there’s always more to the story. Food Safety News missed reporting on the more unique features of Japan’s 1996 outbreak, which was documented as 22 separate outbreaks between May and October (there were a total of 7 E.coli outbreaks in the previous five years): “In July..a large outbreak of exclusively Stx-1 producingE.coli 0118:H2 occurred in a junior highschool in Komatsu city. This appears to be the first report in the world of clinical infection caused by this organism… 241 [students and staff] were symptomatic… Although Stx-producing E.coli was not isolated from the samples of food and water, it was isolated from a dipper… No subjects developed infection at 11 other schools that had served the same..foods… the source of the primary infection was not identified..” One month after that another type of E.coli, O26:H11, that also anomalously produced only the Stx-1 variant of shiga-toxin (again a world’s first), caused a small outbreak. http://pediatrics.aappublications.org/content/103/1/e2.full
Evidently, something very unusual was going on.
*
Stx1 is now a promising treatment for breast cancer: [2009] “The high specificity and apoptosis-inducing properties of verotoxin-1 indicates that the toxin potentially may be used for treatment of  Gb3-expressing breast cancer….Gb3 expression was detected in the vascular endothelial cells of..tumor specimens…” http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650710/ >>>Gb3 is the glycolipid cell-surface receptor for Stx.
*
    What does not seem to have been recorded in the huge wave of Japan’s pathogenic E.coli that year was the presence of O104 E.coli.  The earliest mention of an outbreak of an O104 strain anywhere that turns up in the literature happened in Montana:  “During February-March 1994…Eleven confirmed and seven suspected case-patients were identified..” with a “rare serotype E.coli O104:H21 that produced Shiga-like toxin II” (Stx2-like, or SLTII) http://www.cdc.gov/mmwr/PDF/wk/mm4427.pdf
                                                       _______________________________
News from June 23 put the E.coli toll in Germany at 3,533; 39 deaths, 810 cases of hemolytic uremic syndrome (HUS) and 2, 684 less acute cases. http://articles.latimes.com/2011/jun/23/news/la-heb-ecoli-strain-pathogen-20110623; a same day report notes 5 confirmed cases in the U.S. of a “matching strain” and one suspected case resulting in death. The five were recent visitors to Germany: http://www.ecoliblog.com/e-coli-outbreaks/german-e-coli-outbreak-linked-to-massachusetts-michigan-wisconsin-north-carolina-and-arizona/ [updated June 30 to over 4,200 cases, 50 deaths, 898 HUS]
Something in the air?
“There seems to be little or no published work on the inhalation toxicity of Shiga toxin. However, there are often indirect effects on the lungs when the toxin is taken in as a food contaminant.” http://www.cbwinfo.com/Biological/Toxins/Verotox.html; “E.coli can grow in the air, using oxygen as a terminal electron acceptor..” http://www.ecoliblog.com/e-coli-watch/what-is-e-coli-1/
*
 “Ricin and Shiga toxin share a unique mode of action, causing irreversible damage to host ribosomal RNA. Our understanding of how these bioterror agents cause tissue damage is incomplete… the mechanism(s) by which these signal transduction pathways are stimulated will be assessed using Shiga toxin mutants and isolated Shiga toxin subunits.” [grant award to Tufts Medical Center by U.S. Dept of HHS] http://projectreporter.nih.gov/project_info_description.cfm?projectnumber=5R01AI059509-04
*
   Food and water are always the assumed vectors for E.coli contamination but if lung ‘effects’ from ingestion of the more toxic (HUS-causing) particle, Stx2, are indistinguishable from inhaled Shiga toxin then the Lords of the Air could whip up another perfect storm and blame it on ‘behavior’. The data on Stx (shiga-toxin) inhalation is currently similar to, and perhaps less than, the knowledge-ceiling of anthrax before the post 9-11 attack, given that no aerosol attack of Stx2 has ever happened before. At least no registered terror groups have ever launched a shiga toxin attack, but in case they do…well, the establishment looks almost prepared. Real time experience with countermeasures afforded by this new opportunity should be a good lesson, after all, the outbreak is erupting under the watchful eye of major defense contractors.
                                                      _______________________________
                                                      _______________________________
The new O104 strain is “not new” according to the CDC and “There is no reason to think that this strain was modified intentionally… The combination of Shiga toxin and enteroaggregative features has been seen before; 1) in E.coli O104 identified in Europe and Asia in the last decade, and 2) in a different strain of E.coli that caused a small outbreak in Europe in the 1990s. While this combination is uncommon, it is not new.” http://www.cdc.gov/nczved/divisions/dfbmd/diseases/ecoli_o157h7/index.html#difference
   How new does new have to be? Toxic E.coli is new and became a reportable pathogen in 1982. Speculation about its emergence suggests it may have appeared in the 1950s. Looking at the volume of studies and papers on toxic E.coli generated at Harvard, for example, proves just how new the science is. There is nothing before 1997 and presumably no public funding either. Students and staff just don’t work on ‘unimportant’ diseases. http://connects.catalyst.harvard.edu/profiles/profile/concept/Escherichia+coli+O157
To wit : [2001] “Shiga toxin-producing E.coli (STEC) such as E.coli O157:H7 are important emerging pathogens in the United States… The genes encoding these potent toxins are present on viral particles known as bacteriophages… treating them with certain antibiotics leads to an increase in toxin expression. The antibiotics also lead to increased movement of the viruses…” http://www.reeis.usda.gov/web/crisprojectpages/192539.html; that’s called a bacterial “SOS” response, and a warning not to self-medicate with antibiotics –read the Colloidal Silver post too;  natural garlic ‘allicin’ has shown to be effective against shiga toxin bacteria.
*
Current CDC estimates for Stx-producing E.coli infections in the U.S. stand at 186,000 cases annually http://www.marlerblog.com/lawyer-oped/non-o157-e-coli-in-beef—how-does-fsis-justify-it/; up from approx. 110,000 annually a decade ago.
*
Where did  Shiga toxin come from?
   It was first isolated from an E.coli-like bacterium in 1896 Japan, and named Shigella for its discoverer, Kiyoshi Shiga. Four types of Shigella bacteria, all causes of dysentery (Shigellosis), have been characterized: Shigella dysenteriae, Shigella flexneri, Shigella boydii and Shigella sonnei, the most complex of which is Shigella flexneri, isolated by Simon Flexner c.1900 in the Philippines. Among the four types, S.flexneri toxin induced neurological damage and most often led to kidney failure and death. Flexner soon became the founding director of the Rockefeller Institute for Medical Research (RIMR) in New York. http://polioforever.wordpress.com/the-rockefeller-institute/
*
The shiga toxin that is spreading in E.coli nowadays is carried by the lambda** phage, a viral particle and the most particularly interesting-to-science component of E.coli bacteria that has opened the secrets of genetic transference. Lambda phage was discovered by Esther Zimmer Lederberg, the wife of Joshua Lederberg, in 1950. http://www.estherlederberg.com/LambdaP.html
**”Lambda..is the 11th letter of the Greek alphabet…related to..the Cyrillic letter ‘El’..  http://en.wikipedia.org/wiki/Lambda
*
 …”If the devil were to work overtime in a plot to confound scientists in their quest for a causative agent to a serious disease of unknown origin, it is doubtful whether he could have come up with anything better than the phage…”
 Head I        Head II
Joshua Lederberg, a Rockefeller University president and National Science administrator, is cited in this blog as the editor of a collected genetics publication about mutation: https://jenniferlake.wordpress.com/2009/08/11/mutation/ ; http://en.wikipedia.org/wiki/Joshua_Lederberg  In 1946, Joshua Lederberg codiscovered bacterial conjugation, the process credited to transferring Shiga toxins to E.coli.  He was also the principal coordinator of the first “artificial intelligence” project applied to chemobiology – the mid-60s DENDRAL super-computer at Stanford which set the groundwork for current Nano-Cogno-Bio-Information systems that plot “beyond conception” formulas for artificial life creation. Lederberg styled himself as an exobiologist. http://profiles.nlm.nih.gov/BB/Views/Exhibit/narrative/ai.html
*
“From 1979 to the present, Lederberg has been a Trustee of the Sackler Medical School at Tel-Aviv University, and, since 1990, has been a Beverly Sackler Foundation Scholar at Rockefeller University. In 1994, he headed the Defense Department Task Force on Persian Gulf War Health Effects, “which concludes that there is insufficient epidemiological evidence for a coherent Gulf War
Syndrome.” http://www.smokershistory.com/Lederber.htm
“..as chairman of the..Task Force on Persian Gulf War Health Effects.. Lederberg was also on the board of directors of the American Type Culture Collection, or ATCC…[that] made 70 government-approved shipments of anthrax..to Iraqi scientists..” http://chss.montclair.edu/english/furr/pol/lederber.html
*
Bacteriophage lambda has been used as a host vector for [the] generation of genomic..libraries for quite some time; ..generated primarily because of the phage particles’ ability to efficiency infect bacteria in vitro [lab dish]. Some research workers prefer to use the original lambda as a vector while others..like to propagate the gene fragments in a cosmid vector.” http://www.biocompare.com/Articles/ProductReview/867/Packagene-Lambda-DNA-Packaging-System-From-Promega.html
   The link below is a patent that promises a lambda vector system that can deliver recombinant DNA into the mitochondria of mammalian cells http://www.freepatentsonline.com/7741112.html; the lambda “taxi” can be virtually stripped of its own genetic material and used to import any select package of genes to almost any cell of choice, including genes that code for specific “receptors” to a range of viruses. These techniques offer the treatment paradigm of “virotherapy”, where viruses deliver genetic ‘correction’ to targeted cells.
    For a long time, antibiotics served as “tracers” in recombination studies by inducing resistance in the objects of study which could thrive and be isolated from cultures that were otherwise poisoned by an antibiotic– thus, not only do cellular entities accumulate antibiotic resistance as a type of mutation, but so do their interchangeable parts,  compounding their antibiotic-resistance profiles. For example, a citation from a Finnish study covering infections reported 1990 to 2000 notes E.coli “sensitivity to 12 antimicrobial agents.http://www.ncbi.nlm.nih.gov/pmc/PMC88246 .  State-of-the-art recombination genetics is readily taking up “marker” protein genes like the green fluorescent protein (gfp) from jellyfish, ostensibly as a safer alternative and infinitely fascinating as another paradigm in transgenetics. Still, antibiotics are very useful genetic expression ‘promoters’ and continue to be used in bioengineering.
*
The toxin-making parts of lambda-bearing genomes have all been isolated and described but scientists have yet to realize the fullness of biochemical conditions that cause the toxin genes to turn on and ‘express’. They do know that “the bacteriophage life cycle is the dominant, if not the only, important factor involved in stx2 expression.” http://www.ncbi.nlm.nih.gov/pmc/PMC95105   Why would (some) scientists want to turn on the deadly shiga toxin-producing mechanism of the genes? There are reasons far beyond the call of medicine. Genetic engineers have very sophisticated needs in their search for knowledge and limited technologies and resources with which to explore them. I imagine there’s nothing quite as effective as handing a major public health threat over to a technically advanced society in order to get some help. The shiga toxin components of genes have enormously useful properties in the genetics industry.
*
   Here’s a thought about weaponeering: “The [Stx] protein is robust, stable, relatively easy to manufacture and has been the subject of intense research over many years…This [body of] information..may also be used to develop novel variants of greater effectiveness. The protein does not act through the skin, and effective protection can probably be obtained with a gas mask with activated charcoal filters.” http://www.cbwinfo.com/Biological/Toxins/Verotox.html
                                                     ________________________________
                                                        _____________________________
                                                                     CHANGE AGENTS
E.coli research has been a long-standing field of study. ..”The bacterium Escherichia coli is truly the workhorse of biology. Originally isolated in 1922 from a diphtheria patient, the strain of E.coli [completely and recently] sequenced  was used in 1945 in the discovery of spontaneous gene transfer. The strain, known as K-12, was universally adopted for fundamental work in biochemistry, genetics and physiology…E.coli is not the first bacterial genome to be sequenced, but with more than 4.6 million bases, it is the largest and possibly most important… The K-12 strain of E.coli is not pathogenic but closely related strains are toxic.” http://accessexcellence.org/WN/SUA11/ecoli997.php .  Mentioned earlier in “Transgenic Round-up”, recombinant DNA techniques were perfected by the 1970s with E.coli. See the work of Paul Berg and Maxine Singer.
   In the last decade E.coli research has opened a biotechnology platform for wholly new ‘life’ creations with artificial amino acids:
[2001] “Expanding the Genetic Code of Escherichia coli” [from the Scripps Research Institute laboratory of Peter Schultz and Salk Institute fellow Lei Wang] http://www.sciencemag.org/content/292/5516/498.abstract
“..We have evolved [a bioactive agent]..that makes possible the in vivo incorporation of [an artificial synthetic amino acid] into proteins in Escherichia coli… This unnatural amino acid was incorporated with high translational efficiency and fidelity.” http://www.ncbi.nlm.gov/pmc/articles/PMC123203/
“Augmenting proteins with unnatural amino acids could make existing proteins more potent in their actions, or even endow them with entirely new properties that might be useful for industry or medicine. Dr. Schultz’s [E.coli] bacteria, for example, look and act entirely like normal creatures until they are placed in contact with a certain sort of poison. Then, because they are producing an unnatural protein, they survive while all the others die.” http://www.economist.com/node/987697?story_id=987697
“..multiple unnatural amino acids can be added to the genetic code of a single modified organism.”  http://www.sciencedaily.com/releases/2004/05/040512040149.htm
*
   Raising the ‘possibilities of meaning’ in E.coli outbreaks occurring after 2004, when the ‘new amino’ insertion techniques became a commodious reality, deserve special attention. Two considerations come to mind; that the E.coli are stealthily vectoring artificial genes and the shiga toxin subunits are installing genetic controls: one vector with multi-use potential. It will take months and years for published study material on the current outbreak strain to make it into the public sphere, even then, the likelihood that new amino acids are factored into standard tests is slim-to-none. For the time being, only the upper echelon labs will have the capacity to identify the rogue material. General researchers fully understand that mutant strains present unknowns which can remain unknown or evade definition for years. If such a probability exists at present, it’s a secret experiment that will bear fruit for its instigators who have in their labs a description of ideal-sounding binary bioweapons. The close structural relationship and gene-swapping properties of E.coli and related “homologous” phage is a biological SureThing, spreading naturally wherever it is
introduced (albeit slowly unless unnaturally assisted).  Stx-producing enterohemorrhagic E.coli (STEC) is also a sure thing– sure to drive victims to seek emergency medical care. There’s going to be lots of samples, lots of DNA, and probable new toxic mutants.
*
“Physicians know not to prescribe antibiotics for O157 infections because the sudden killing of the bacteria can release HUS[hemolytic uremic syndrome]-inducing and potentially deadly amounts of Shiga toxin… that fact could have created one major problem in the early development of the [May 2011] outbreak: It is likely that German hospitals were only screening the first enterohemorrhagic E.coli symptoms for O157 and not O104, which no one would have suspected… ‘In this case, [said Jorge Giron], because it wasn’t O157, the physicians might have thought it was okay to give antibiotics, not knowing that O104 would produce the Shiga toxin… This potential misunderstanding over antibiotics might at least partially explain the high rate of HUS among the ill’ ” http://www.lavidalocavore.org/diary/4763/o104h4-may-change-how-we-deal-with-e-coli >>It might because somehow the unprecedented rate of HUS needs explaining. Previous toxic E.coli outbreaks have produced a rate of HUS around 3-6%, now trending upwards to 10%.  But just as likely to happen only moreso among skilled physicians who know they don’t have O157 on their hands is to give no antibiotics and allow the normally self-limiting infection to run its course– this is especially plausible with adult patients who subsequently made up the majority cases of HUS.  The 1996 Komatsu Japan Stx1 school outbreak (non-O157), linked above, is an example of infected patients receiving no antibiotics (approx. half) and resolving their infections equally well and sometimes better. Mr. Giron’s suggestion just doesn’t fly. The more I look at the German “Egyptian sprout” outbreak, the stranger it gets in comparison to other documented outbreaks. I’m considering the cofactor with radioactive fallout based on past anthrax episodes as an opportune moment-in-time, so I’ve set up a link with microbe-hunting results and I’m adding in the accumulating news.
*
Hemolytic uremic syndrome is a severe, life-threatening complication of an E.coli..infection that was first described in 1955… The Shiga toxin triggers a complex cascade of changes in the blood..and there is disruption of the inherent clot-breaking mechanisms… About ten percent of individuals with [toxic] E.coli..infections (mostly young children) goes on to develop Hemolytic Uremic Syndrome.” http://www.marlerblog.com/legal-cases/three-suffering-kidney-failure-hus-from-alabama-e-coli-poisoning/; “The principle organ affected in STEC-mediated HUS is the kidney… The severity of renal injury varies in degree from urinary abnormalities such as hematuria and proteinuria to acute renal failure… The second most important organ affected in the disease is the brain… more serious cerebral complications, including seizures, cortical blindness, and thrombotic strokes, occur in 5 to 10% of patients… A similar percentage of patients may develop life-threatening cardiopulmonary sequelae, including adult [acute] respiratory distress syndrome [ARDS], congestive heart failure and myocarditis. The occurrance of neurological and cardiovascular complications is associated with more severe STEC-induced HUS and a higher risk of mortality during the acute illness. Other organs that are frequently involved in STEC-induced HUS are the endocrine and exocrine pancreas, liver, gall bladder, gastrointestinal tract, and skin. It is evident that STEC-induced HUS is a systemic illness potentially affecting every organ throughout the body.http://cmr.asm.org/cgi/content/full/17/4/926
*
“…the outbreak originating in Germany shows 87% of those hit by the disease were women over the age of 20… the German outbreak..displayed ‘several intriguing microbiological characteristics’ and ‘several novel epidemiological and clinical features’..[that] ‘differed remarkably’ from previously described STEC/VTEC infections… Among the ‘completely unexpected’ features was the development of severe neurological symptoms in half of patients within 3 to 10 days of being admitted to hospital..” http://www.irishexaminer.com/ireland/kfojidqlsney/rss2/
*
                                                No treatment for humans — vaccines for cows
More information about Shiga toxin E. coli, Alexion* Pharmaceuticals Inc. (et.al.) and the bioweapon potential of Stx is here  http://citizen2009.wordpress.com/e-coli/ (page in progress) and keep this in mind:
“Biotechnology now allows us to genetically engineer animals so that they produce proteins that are human pharmaceuticals. For certain drugs that are difficult to produce using existing methods or are needed in large quantities, production in GE animals offers the most efficient and practical solution. In the case of fighting infectious diseases, GE animal-made antibodies can be produced from animals that have had the human antibody genes transferred to them. These animals can then be vaccinated against human diseases and antibodies can be collected from their blood and used for treating diseases in humans. For example, antibodies can treat infections that are resistant to antibiotics.” http://www.bio.org/foodag/animals/GE_An_Pharm_0908.pdf
“On March 13, 2009, the USDA granted a conditional license for a new tool in the battle against foodborne illness –a vaccine for cows to prevent infection with E.coli bacteria… Cattle themselves do not experience illness from the bacteria. ” http://www.dmaonline.org/CE/food_protection/2009_05.shtml
>>>cattle immunity to STEC  no longer seems to be true –another significant round of ‘firsts’ in 1996 involves cattle infections.
*
   Alexion Antibody Technologies was invited to give Congress a very exclusive pitch for Project BioShield funds in 2003, before approval of the program, and now the German health authorities have approved of Alexion running clinical drug trials on the latest E.coli victims. Alexion is giving away an ultra-expensive drug in exchange for access to patients.
*The nuclear power company UniStar bought Alexion Pharmaceuticals’ property in 2007; http://maryland.realestaterama.com/2007/12/19/unistar-properties-defies-slow-real-estate-market-with-purchase-of-311-million-bioscience-facility-ID041.html; does the relationship get cozier? “Arch Chemicals” also shares the site. There must be some advantage in having “the world’s largest owner and operator of nuclear energy facilities” as a landlord. http://unistarnuclear.com/; UniStar was taken over in 2010 by Electricite de France (EdF). “Both EdF and Areva are backed by the French state.” http://www.world-nuclear-news.org/C_EdF_takes_control_of_Unistar_2710101.html
*
[Feb 2011] Alexion Chairman Max Link sold 47,367 shares, valued at $95 per share… “he retains 92,898 shares… Dr. Link has been the company chairman since December 2002..[and was] formerly CEO of Corange, the parent company of Boehringer Mannheim Therapeutics..[also] formerly the Chairman and CEO of Sandoz Pharma, Ltd.”…”Ann M. Veneman was appointed to Alexion’s Board..previously she served as Executive Director of the United Nations Children’s Fund (UNICEF)”..[from 2005-2010, after resigning her GWBush appointment as Secretary of Agriculture]
http://newyork.citybizlist.com/18/2011/2/25/Chairman-Max-Link-Sells-4.5M-in-Alexion-Shares–cbl.aspx;
Dr. Max Link also became Chairman of Protein Design Labs Inc.[Fremont,CA] in 2004, when the developer of antibody drugs accrued major revenues from products like Avastin (licensed to Roche subsidiary Genentech): “Avastin is a recombinant humanized monoclonal IgG1 antibody..of mouse origin..produced in a Chinese hamster ovary..” approved in 2004 for colorectal cancer treatment. http://www.discoverymedicine.com/Benjamin-Yang/2009/06/17/drug-profile-avastin
 “Ms. Veneman’s training and experience as a corporate lawyer for agribusiness do not qualify her for the substantial task of leading the agency most responsible for the rights of children worldwide. There is no evidence in her tenure as U.S. secretary of agriculture, secretary of the California Department of Food and Agriculture, or deputy undersecretary for international affairs of the USDA of her interest in the world’s children or their health and well-being.” http://www.sourcewatch.org/index.php?title=Ann_Veneman
   In addition to Alexion Pharmaceuticals, Veneman joined the Board of Nestle, the world’s largest food and beverage producer: “Veneman took the post despite pleas from nutrition advocates who urged her not to lend her imprimatur to the company’s marketing of breast milk substitutes” http://www.newsok.com/ex-unicef-head-joins-nestle-board-despite-protests/article/feed/250068?custom_click=pod_headline_europe; the best preventive to Stx-producing E.coli in children is, naturally, breast milk. In Europe and the U.S., 1971 marked an all-time-low in breast-feeding when only 1% of new mothers chose to persist in natural feeding past their ‘hospital stay’. The successful War on Milk rages on…
*
                                                                Deadlier Threshold for Stx
Researchers recently learned that Stx genes multiply exponentially faster than their E.coli hosts:
 [2007] “Demonstration of the ability of [ Stx phage] to form multiple lysogens has two potentially serious impacts. First, multiple integrated prophages will drive the evolution of bacterial pathogens as novel Stx-phages emerge following intracellular mutation/recombination events. Second, multiple copies of the Stx gene may lead to an increase in toxin production and consequently increased virulence…. there is considerable scope for Stx integration directed by as yet uncharacterized factors… Clearly, the occurence of multiple lysogens in a single host [cell] is likely to enhance the evolution and dissemination of bacteriophage-encoded genes throughout bacterial populations, with particular applied relevance for Stx-phages responsible for increasing the pathogenic potential of Escherichia coli hosts.”  http://mic.sgmjournals.org/content/153/12/4098.full
SHIGA-BOOM!
                                                      ______________________________

May 11, 2011

The Minimal Genome Project

“Since the early days of molecular biology, the search for the minimal genome has been the ‘Holy Grail’..”
“The search for the ‘smallest autonomous self-replicating entity,’ which subsequently became the search for the smallest cell genome, was begun in the late 1950s by Harold Morowitz… This led to studies of the mycoplasmas, showing that these microorganisms have the smallest reported cell and genome sizes [as of 1996]. The DNA sequence of the smallest known mycoplasma genome, that of Mycoplasma genitalium, recently was determined… The nature of selective pressure for repeated genome reductions..is not known..[but] have been suggested to be due to selection for faster (hence, smaller) replicating genomes to produce greater progeny..yields, selection for smaller genomes to reduce the energy burden..in limiting nutrient environments, and loss..[due to] deleterious mutations… Since the early days of molecular biology, the search for the minimal genome has been the ‘Holy Grail’… subtracting the minimal gene set from an organism’s total gene inventory should reveal genes for the phenotype characters that make each organism unique.” http://www.sbs.utexas.edu/psaxena/bio226r/articles/minimum_cell_genome.pdf
Harold J. Morowitz:
Oral History Interview, March 16, 2005 — “This interview chronicles Morowitz’s scientific career in detail, beginning with his education in physics, his transition to biophysics, to his ongoing attempt to apply..information theory to..biological problems. His description..gives a valuable insight of how and why physicists after WWII came to be interested in..biological problems…[and] illustrates the reciprocal interaction between scientific projects..in Yales’s biophysics program..and the Atomic Energy Commission’s promotion of the peaceful use of atomic energy (e.g. nuclear fallout debate)..”
                                                          ____________________________

Remember SARS in 2003? Looking back on SARS led me to something dubbed the Minimal Genome Project which I found a lot of experimental documentation for between 1998 and 2000, including the creation of completely new amino acids –until this, every biological entity that has ever existed used the same available 20 amino acids. SARS patients were found to be infected with a novel corona virus and, coincidently, a novel corona virus was created in a lab with a new amino acid in 2001. Since the original outbreak, the SARS creature seems to have disappeared. Was it part of an experiment to create a new “minimal genome organism” ?  Indirect evidence is tantalizingly suggestive that it could be the case.  First, consider this research news in the right time window, and I’ll add more to this post supporting a proof-of-concept. https://jenniferlake.wordpress.com/2009/12/01/sweating-the-small-stuff/ This link, http://jvi.asm.org/cgi/content/abstract/74/22/10600 within Sweating the Small Stuff states that coronavirus is the largest known genome in nature –ripe for a take-away project, I suppose. Since the 2003 outbreak new information about coronavirus is being published, such as this statement from August 2004: “The coronavirus replicase-transcriptase was recently predicted to contain RNA-processing enzymes that are extremely rare or absent in other RNA viruses” –in other words, the researchers have made a novel discovery about their specimen. In this case, the studied coronavirus is generating a unique protein that has an ability to preferentially cleave double stranded RNA (dsRNA):  http://www.ncbi.nlm.nih.gov/pmc/articles/PMC514660/ . This feature of the SARS virus is creating a handy new enzyme tool for genetic engineers.

Minimal genomes are a boon to living DNA computers:“Human cells and computers process and store information in much the same way… ‘If you look inside the cell, you find a bunch of amazing little tools,’ said Adelman, who made the first DNA-based computation in 1994. ‘The cell is a treasure chest.’ ” http://www.cbsnews.com/stories/2003/08/18/tech/main568893.shtml; Leonard Adelman is employed by George Mason University and the MITRE Corporation, producer of the JASON reports for the DoE, DoD, etc.; Leonard Adelman says “Late one evening, while lying in bed reading Watson’s text, I came to a description of DNA polymerase. This is the king of enzymes – the maker of life. Under appropriate conditions, given a strand of DNA, DNA polymerase produces a second “Watson-Crick” complementary strand, in which every C is replaced by a G, every G by a C, every A by a T and every T by an A. For example, given a molecule with the sequence CATGTC, DNA polymerase will produce a new molecule with the sequence GTACAG. The polymerase enables DNA to reproduce, which in turn allows cells to reproduce and ultimately allows you to reproduce. For a strict reductionist, the replication of DNA by DNA polymerase is what life is all about… DNA polymerase is an amazing little nanomachine, a single molecule that “hops” onto a strand of DNA and slides along it, “reading” each base it passes and “writing” its complement onto a new, growing DNA strand.  http://www-gap.dcs.st-and.ac.uk/~history/Biographies/Adleman.html; http://www.genealogy.math.ndsu.nodak.edu/id.php?id=62298 ; simpler genomes are helping this process along. Adelman’s counterpart in Israel, Ehud Shapiro, works on a project at the Weizmann Institute called “The Human Cell Lineage Tree** Flagship Initiative” http://www.wisdom.weizmann.ac.il/~udi/ An accomplishment of Shapiro and colleagues in 2004 illustrates a DNA computer at work: “Recently, simple molecular-scale autonomous programmable computers were demonstrated..allowing both input and output…. Such computers, using biological molecues as input data and bilogically active molecules as outputs, could produce a system for ‘logical’ control of biological processes… As proof of principle we programmed the computer to identify and analyse mRNA of disease-related genes associated with models of..cancer, and to produce a single-stranded DNA molecule after an anticancer drug.” http://www.nature.com/nature/journal/v429/n6990/abs/nature02551.html

There it is: cell surveillance and cancer ‘self’ control.

With DNA polymerase to “process”, restriction enzymes to “cut” and ribozymes to “paste”, the working parts of DNA computers look to be on hand with the exception maybe of genetically stable, high-fidelity replicators. http://www.jci.org/articles/view/19386
I speculate that cancer cells are very useful for this purpose with their immortal qualities, and in so many cases, engineered recombinant microbes (i.e. viruses) in current therapy use end up causing cancers, that the cancer cells, and controlling the cells, in the first place is the most useful approach. Minimal genomes, real and artificial, look like other lines of pursuit running apace for the ultimate creation of DNA computer life. This general field of study is called bioinformatics http://www.med.nyu.edu/rcr/Fordham/ and another of its Holy Grails is finding perfect cell-based delivery systems. The DNA computer ‘proof of principle’ by the Israelis (above) used a “stochastic molecular automaton” to provide the computer input, meaning a precise-targeting agent –I’m perusing the literature to find the exact agents– but this fundamentally defines the action of viruses. And the genetically small ones, like SV40 monkey virus and polioviruses are already human-adapted and under intense ‘quantitative’ experimentation to see how well they deliver genetic information.
*
**Human Cell Lineage Tree Project collaborators in the U.S. are Shimon Weiss of UCLA http://www.chem.ucla.edu/dept/Faculty/sweiss/  and Stephen Quake at *Stanford http://www.hhmi.org/news/quake.html; for more ‘flagship’ programs see this http://cordis.europa.eu/fp7/ict/programme/fet/flagship/doc/pilot-papers-01_en.pdf
*

____________________________________

Chalk this up to spooky things scientists say.
November 22, 2002 — “Craig Venter’s ‘minimal genome’ project announced Wednesday is not about creating a new life form…[that comes later with Synthia]. The high profile project was just funded by the U.S. Department of Energy (DoE) with $3 million going to the Institute for Biological Energy Alternatives (IBEA), one of the nonprofit research institutes Venter founded after leaving..Celera Genomics early this year.
   “The question of the minimal genome for an organism [–the base gene set required for life–] is always ‘minimal in which environment,’ said Francisco J. Silva of the University of Valencia in Spain. Silva and colleagues..are studying the genome of the insect endosymbiont Buchnera aphidicola, which appears to have an even smaller genome than that of the parasite Mycoplasma genitalium, Venter’s organism of choice.
   “..pathogenic bacteria usually have more genes than their harmless relatives do..but the disease-related genes are not essential to life, so they could be the first catagory of genes a scientist would jettison from a minimal genome organism… ‘A minimal genome organism’ [Silva] said, ‘will be a prisoner of the laboratory dish where it lives, and will be unable to compete with the outer world.’
   “The minimal genome organism now being planned..will be further crippled so that it cannot survive wihout laboratory coddling. The strategy is to synthesize an artificial chromosome containing the presumptive minimal gene set, remove all existing genetic material.. and then insert the synthetic chromosome into the vacant cell… The long-term goal..will be to help the world solve some of its environmental problems. That’s why the funding is coming from DoE, where recent genetics projects have focused on bioremediation…” http://www.the-scientist.com/article/display/20885/
>>>In June 2010, Venter announced “Synthia” –“a synthetic cell from scratch” and “a hitherto unseen lifeform…To do this, he read the DNA of Mycoplasma mycoides, a bug that infects goats, and recreated it piece by piece. The fragments were then ‘stitched together’ and inserted into a bacterium of a different species… he claimed the breakthrough had changed his views on the definition of life. ‘We have..the first synthetic cell powered and controlled by a synthetic chromosome and made from four bottles of chemicals,’ he said..” http://www.dailymail.co.uk/sciencetech/article-1279988/Artificial-life-created-Craig-Venter–wipe-humanity.html
*
“The constraints of the genetic code are history”
Feb. 14, 2002 – ” ‘The constraints of the genetic code are history,’ proclaims Peter Schultz of the Scripps Research Institute in La Jolla, California…’At least in bacteria, the genetic constraints..are gone.’ Dr. Schultz’s statement is no idle boast…[His] laboratory, along with another team of chemists based in Japan, are introducing completely new strands [of DNA]…If successful, they will fabricate..a new sort of living thing… [C]olleagues..in..Japan..have developed unnatural versions of all the ingredients in this process: the bases, the DNA, the RNAs and the amino acids. The result is a protein that could never have existed in nature… By exposing..unnatural bacteria to the sort of conditions believed to have prevailed on the early earth..it might be possible to observe whether bacteria with 21, 22, or even more amino acids might win out over those with the standard complement… Back in the present, the next order of business is to apply the research to mammals. Dr. Wang says that a certain strain of monkey cells has shown a promising tendency to incorporate unnatural amino acids. Within a year, he thinks, the group should have made mammalian cells equipped with 21 amino acids.” http://www.economist.com/node/987697?story_id=987697
>>>Dr. Lei Wang is on the faculty of the Salk Institute http://www.salk.edu/faculty/wang.html; it certainly seems like the Schultz team was eager to know if their creations would ‘compete’ outside the lab.
   In July 2002, Eckard Wimmer of SUNY, in conjunction with the Pentagon, announced the result of his DARPA-funded project to create viruses from scratch:
“This is the first time that a working biological entity has been made using chemicals alone…But poliovirus is easier to build than many others. It has a short and simple genome and assembles itself directly from a DNA template… More complex viruses could be synthesized, Wimmer believes, by additional chemical steps or by putting synthetic genes into living cells..” http://www.nature.com/news/1998/020708/full/news020708-17.html
Commenting on Wimmer’s achievement: ” ‘This work should never have been done, funded, or published,’ said J.Craig Venter…’Somehow the whole system broke down here.’ ..The work, [Wimmer] said, was intended to serve as a warning of what is now possible.”
2004— “Several attempts have been made to identify the minimal set of genes that is required for life using computational approaches… These experiments resemble those already performed by nature; a few hundred million years ago an ancestor of E. coli was domesticated by aphids which resulted in the elimination of 70-75% of the original bacterial genome. Amazingly, the small genomes of the imprisoned bacteria are more stable than those of their free-living relatives.
   ..”Obligate host-associated bacteria have among the smallest genomes known in nature… Two different scenarios have been proposed to explain the process of genome shrinkage in B. aphidicola… that the minimization..was continuous, with genes being lost individually through a large number of small deletion events..[or] that many genes were lost simultaneously at an early stage of the internalization process..through the elimination of large blocks of DNA spanning multiple genes…
 …”as computational analysis becomes more refined and the data sets grow larger, fewer genes remain that are conserved among all taxa…[and] the few remaining genes are organized in a surprisingly similar manner… The lack of..sequences..reduces rearrangement possibilities…
   “The next few years will tell whether..the..endosymbiont genomes are self-sustainable… An interesting question for the future is to determine whether the dependent partner will be ‘allowed’ to stay as a ‘silent parasite’ if the need for its functions is lost during evolution, or if such a loss will cause it to deteriorate and collapse.”
Error Catastrophe
Too many mutations, too rapid a pace and/or too few genes leads to a potential species collapse in a  nose-diving degenerative process that microbe researchers in the lab call “error catastrophe”; virus experimenters document the fundamental principle, noting, “There is an intrinsic limit to the maximum variability of viral genetic information before it loses meaning and if an RNA virus quasispecies goes beyond that mutation limit, the population will no longer be viable. The phenomenon that occurs when the loss of genetic fidelity results in a lethal accumulation of errors has been termed ‘error catastrophe’. Most cellular organisms have evolved a number of sophisticated processes to maintain their genetic information with high fidelity and stay far away from the threshold of error catastrophe. In contrast, it has been predicted that RNA viruses with high mutation frequencies exist close to the edge of error catastrophe and can be forced into error catastrophe by a moderate increase in mutation rate… We also describe direct evidence that the error catastrophe theory applies to poliovirus.” http://www.pnas.org/content/98/12/6895.full
“One very important fact to remember is that radiation increases the spontaneous mutation rate” — Nuclear Regulatory Commission (NRC) power plant training manual on the effects of ionizing radiation
   Another important fact is that viruses are not organisms or cells and have no strategies for survival! The authors of the ‘error catastrophe’ paper are really telling us that the “edge of viability” on which polioviruses (and others) exist needs maintenance to prevent their extinction. Circulating polioviruses were determined in 2000 to be all vaccine-derived, and the buzz around the conference tables of the WHO and other agencies brought up the issue of stopping the inoculation programs. Very soon thereafter
a complete scratch poliovirus made from mail-order chemicals blitzed the science headlines invoking a new threat from terrorists. As a mode of comparison, organisms do have an array of survival strategies.
Next Page »

Blog at WordPress.com.